EP 1157126 A1 20011128 - METHOD FOR CLONING SIGNAL TRANSDUCTION INTERMEDIATES
Title (en)
METHOD FOR CLONING SIGNAL TRANSDUCTION INTERMEDIATES
Title (de)
VERFAHREN ZUM KLONEN VON ZWISCHENPRODUKTEN FÜR SIGNALTRANSDUKTION
Title (fr)
PROCEDE DE CLONAGE DE PRODUITS INTERMEDIAIRES DE TRANSDUCTION DE SIGNAUX
Publication
Application
Priority
- US 0004925 W 20000224
- US 12148599 P 19990224
Abstract (en)
[origin: WO0050633A1] The invention features a method of identifying a polypeptide which increases gene expression from a promoter, including (a) contacting a library of polypeptides with a cell that expresses a recombinant anti-cell death gene and that contains a reporter gene operably linked to a promoter (as shown in the figure), whereby expression of the reporter gene is increased if the library includes a polypeptide which increases gene expression from the promoter; (b) determining whether the reporter gene expression is increased in the cell as a result of contact with the polypeptide library; and (c) if reporter gene expression is increased, identifying a polypeptide of the library which increases reporter gene expression.
IPC 1-7
C12Q 1/00; C12Q 1/70; C12Q 1/66; C12N 7/00; C12N 5/00; C12N 5/02; G01N 33/554; A61K 38/00; C07H 21/02; C07H 21/04; C07H 21/00; C12Q 1/68
IPC 8 full level
A61K 38/00 (2006.01); A61P 29/00 (2006.01); A61P 31/12 (2006.01); A61P 35/00 (2006.01); C07K 14/47 (2006.01); C07K 14/705 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6897 (2018.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/566 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
A61P 29/00 (2017.12); A61P 31/12 (2017.12); A61P 35/00 (2017.12); C12Q 1/6897 (2013.01); G01N 33/5011 (2013.01); G01N 33/6872 (2013.01)
Designated contracting state (EPC)
CH DE FR GB LI
DOCDB simple family (publication)
WO 0050633 A1 20000831; WO 0050633 A9 20011011; AU 3380200 A 20000914; EP 1157126 A1 20011128; EP 1157126 A4 20050302; JP 2002541779 A 20021210
DOCDB simple family (application)
US 0004925 W 20000224; AU 3380200 A 20000224; EP 00911997 A 20000224; JP 2000601196 A 20000224