Global Patent Index - EP 1159400 A1

EP 1159400 A1 20011205 - CULTURE OF MYCOBACTERIA

Title (en)

CULTURE OF MYCOBACTERIA

Title (de)

KULTIVIERUNG VON MYKOBAKTERIEN

Title (fr)

CULTURE DE MYCOBACTERIES

Publication

EP 1159400 A1 20011205 (EN)

Application

EP 00907778 A 20000302

Priority

  • GB 0000760 W 20000302
  • GB 9904773 A 19990302

Abstract (en)

[origin: WO0052139A1] The present invention provides a method of culture of mycobacteria which comprises culturing the mycobacteria, either in batch or continuous culture, with agitation and in the presence of sufficient detergent so that a substantially homogenous suspension of mycobacterial cells is maintained. According to a preferred method the mycobacteria are grown in continuous culture, at a temperature of 35 DEG C +/- 10 DEG C, at a dissolved oxygen tension of at least 1 percent, at a pH of 6.9 +/- 0.9, at a dilution rate of at least 0.02 h<-1> and with agitation in the presence of sufficient detergent to maintain a substantially homogenous suspension of single cells. The present invention also provides a growth medium for culture of mycobacteria, comprising a carbon source, a mitogen, trace elements comprising at least Mg, K, P and S, and a nitrogen source. According to a separate aspect of the invention, there is provided a method of culture of a mycobacteriophage, comprising culture of mycobacteria as described above, and contacting said mycobacteria with a mycobacteriophage.

IPC 1-7

C12N 1/00; C12N 1/20

IPC 8 full level

C12N 1/20 (2006.01)

CPC (source: EP)

C12N 1/20 (2013.01)

Citation (search report)

See references of WO 0052139A1

Citation (examination)

  • MCCARTHY C.M. ET AL: "Continuous culture of Mycobacterium avium limited for ammonia", AM.REV.RESPIR.DIS., vol. 127, 1983, pages 193 - 197
  • JAMES B.W. ET AL: "The physiology and pathogenicity of Mycobacterium tuberculosis grown under controlled conditions in a defined medium", J.APPL.MICROBIOL., vol. 88, 2000, pages 669 - 677

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0052139 A1 20000908; AU 2925500 A 20000921; AU 770840 B2 20040304; CA 2364579 A1 20000908; EP 1159400 A1 20011205; GB 9904773 D0 19990428; JP 2002537798 A 20021112

DOCDB simple family (application)

GB 0000760 W 20000302; AU 2925500 A 20000302; CA 2364579 A 20000302; EP 00907778 A 20000302; GB 9904773 A 19990302; JP 2000602751 A 20000302