EP 1159404 A2 20011205 - EMBRYONIC OR STEM-LIKE CELL LINES PRODUCED BY CROSS SPECIES NUCLEAR TRANSPLANTATION
Title (en)
EMBRYONIC OR STEM-LIKE CELL LINES PRODUCED BY CROSS SPECIES NUCLEAR TRANSPLANTATION
Title (de)
EMBRYONISCHE ZELLLINIEN ODER STAMMZELL-ÄHNLICHE ZELLLINIEN, DIE DURCH KERNTRANSPLANTATION ZWISCHEN VERSCHIEDENEN SPEZIES PRODUZIERT WURDEN
Title (fr)
LIGNEES CELLULAIRES EMBRYONNAIRES OU DE TYPE SOUCHE PRODUITES PAR TRANSPLANTATION NUCLEAIRE D'ESPECES CROISEES
Publication
Application
Priority
- US 0005434 W 20000302
- US 26046899 A 19990302
Abstract (en)
[origin: WO0052145A2] An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Also, methods for improving nuclear transfer efficiency by genetically altering donor cells to inhibit apoptosis, select for a specific cell cycle and/or enhance embryonic growth and development are provided.
IPC 1-7
C12N 5/06; C12N 5/08; C12N 15/87; A61K 35/00; A61P 5/00; A61P 7/00; A61P 9/00; A61P 19/00; A61P 21/00; A61P 25/00; A61P 31/00; A61P 35/00
IPC 8 full level
A61P 5/00 (2006.01); A61P 7/00 (2006.01); A61P 9/00 (2006.01); A61P 19/00 (2006.01); A61P 25/00 (2006.01); A61P 31/00 (2006.01); C12N 15/873 (2010.01); A61K 35/12 (2015.01)
CPC (source: EP)
A61P 1/16 (2017.12); A61P 3/10 (2017.12); A61P 5/00 (2017.12); A61P 7/00 (2017.12); A61P 9/00 (2017.12); A61P 9/14 (2017.12); A61P 11/00 (2017.12); A61P 13/02 (2017.12); A61P 17/02 (2017.12); A61P 19/00 (2017.12); A61P 19/08 (2017.12); A61P 21/00 (2017.12); A61P 25/00 (2017.12); A61P 25/02 (2017.12); A61P 25/14 (2017.12); A61P 25/16 (2017.12); A61P 25/18 (2017.12); A61P 25/28 (2017.12); A61P 31/00 (2017.12); A61P 31/18 (2017.12); A61P 35/00 (2017.12); C12N 15/873 (2013.01); A61K 35/12 (2013.01); C12N 2510/00 (2013.01); C12N 2517/04 (2013.01)
Citation (search report)
See references of WO 0052145A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0052145 A2 20000908; WO 0052145 A3 20010104; AU 3391300 A 20000921; BR 0008714 A 20020102; CA 2364415 A1 20000908; EP 1159404 A2 20011205; IL 145151 A0 20020630; JP 2002537803 A 20021112; NZ 513888 A 20010928
DOCDB simple family (application)
US 0005434 W 20000302; AU 3391300 A 20000302; BR 0008714 A 20000302; CA 2364415 A 20000302; EP 00912131 A 20000302; IL 14515100 A 20000302; JP 2000602757 A 20000302; NZ 51388800 A 20000302