EP 1159414 A1 20011205 - PROCESS FOR ENHANCING ELECTRIC FIELD-MEDIATED DELIVERY OF BIOLOGICAL MATERIALS INTO CELLS
Title (en)
PROCESS FOR ENHANCING ELECTRIC FIELD-MEDIATED DELIVERY OF BIOLOGICAL MATERIALS INTO CELLS
Title (de)
VERFAHREN ZUR STEIGERUNG DER DURCH EIN ELEKTRISCHES FELD VERMITTELTEN EINBRINGUNG BIOLOGISCHEN MATERIALS IN ZELLEN
Title (fr)
PROCEDE SERVANT A PROMOUVOIR L'ADMINISTRATION ASSISTEE PAR CHAMP ELECTRIQUE DE MATERIAUX BIOLOGIQUES DANS DES CELLULES
Publication
Application
Priority
- US 0021982 W 20000925
- US 15564099 P 19990924
Abstract (en)
[origin: WO0123537A1] An object of the invention is to provide simple, nontoxic, and pharmaceutically defined methods for genetic modification of cells and tissues which enable development of a variety of molecular medicines. The invention herein is directed to a method for improving the transfer efficiency of biological material into cells and decreasing the frequency of cell death, in vitro, in vivo, and ex vivo. Specifically, the invention involves the synergistic employment of biopolymer binding reagents, e.g. polynucleotide binding reagents, in combination with electroporation techniques to enhance the efficiency of polynucleotide transfer to the cells of interest and to decrease the frequency of cell death of these cells. The improved electroporation-based gene therapies find particular utility in the transfer of genetic material into cells and has broad applicability for a wide range of gene therapy and polynucleotide vaccine applications. The improved methods of the present invention are useful with both ex vivo and in vivo delivery systems. In addition, the invention herein is not limited to the transport of polynucleotides but rather, is also applicable to the transport of other biopolymers, such as proteins and antigens across cell membranes.
IPC 1-7
IPC 8 full level
C12N 15/82 (2006.01); C12N 15/87 (2006.01); A61K 39/00 (2006.01); A61K 48/00 (2006.01)
CPC (source: EP)
C12N 15/8206 (2013.01); C12N 15/87 (2013.01); A61K 39/00 (2013.01); A61K 48/00 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0123537 A1 20010405; CA 2390716 A1 20010405; EP 1159414 A1 20011205; EP 1159414 A4 20030827
DOCDB simple family (application)
US 0021982 W 20000925; CA 2390716 A 20000925; EP 00964909 A 20000925