Global Patent Index - EP 1159443 A1

EP 1159443 A1 2001-12-05 - METHOD FOR THE IN VITRO TESTING OF ACTIVE INGREDIENTS, CORRESPONDING DEVICE AND THEIR USE

Title (en)

METHOD FOR THE IN VITRO TESTING OF ACTIVE INGREDIENTS, CORRESPONDING DEVICE AND THEIR USE

Title (de)

VERFAHREN ZUM IN VITRO TESTEN VON WIRKSTOFFEN, VORRICHTUNG UND DEREN VERWENDUNG

Title (fr)

PROCEDE POUR TESTER DES PRINCIPES ACTIFS IN VITRO, DISPOSITIF APPROPRIE ET SON UTILISATION

Publication

EP 1159443 A1 (DE)

Application

EP 00907670 A

Priority

  • DE 19910540 A
  • EP 0002011 W

Abstract (en)

[origin: WO0053797A1] The invention relates to a method for the in vitro testing of active ingredients in cells, which includes at least the following steps: provision of a cell culture dish having an inner chamber and an outer wall as well as a first and a second membrane system positioned in the inner chamber, a cell culture chamber being configured between the membrane systems and the inner wall of the inner chamber; introduction of a cell culture and a cell culture medium into the cell culture chamber; addition of a liquid nutrient medium to the cell culture chamber; removal of products of metabolism by means of the first membrane system; delivery of at least one gaseous medium to the cell culture chamber by means of the second membrane system; addition of at least one active ingredient into the cell culture chamber in accordance with a set active- ingredient concentration-time curve; and monitoring of cell vitality. The invention also relates to a device and to the use of said device for testing the effect of idarubicin on the leukaemic cell line CCRF CEM.

IPC 1-7 (main, further and additional classification)

C12Q 1/02; C12M 3/06

IPC 8 full level (invention and additional information)

C12M 3/06 (2006.01); C12Q 1/02 (2006.01); G01N 33/50 (2006.01)

CPC (invention and additional information)

G01N 33/5014 (2013.01); C12M 29/04 (2013.01); C12M 41/48 (2013.01); C12Q 1/025 (2013.01); G01N 33/5008 (2013.01); G01N 33/5044 (2013.01)

Citation (search report)

See references of WO 0053797A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0053797 A1 20000914; EP 1159443 A1 20011205; JP 2002537851 A 20021112

INPADOC legal status


2004-07-28 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20040119

2003-08-27 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030708

2001-12-05 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010816

2001-12-05 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE