Global Patent Index - EP 1161142 A4

EP 1161142 A4 20030122 - METHODS FOR CULTURING FLUID-FILLED SENSORY ORGANS

Title (en)

METHODS FOR CULTURING FLUID-FILLED SENSORY ORGANS

Title (de)

KULTURVERFAHREN FÜR FLÜSSIGKEITSGEFÜLLTE SENSORISCHE ORGANE

Title (fr)

PROCEDE DE CULTURE D'ORGANES SENSORIELS REMPLIS DE FLUIDES

Publication

EP 1161142 A4 20030122 (EN)

Application

EP 00914825 A 20000303

Priority

  • US 0005736 W 20000303
  • US 12310099 P 19990305

Abstract (en)

[origin: WO0054583A1] The present invention provides methods for culturing fluid-filled sensory organs in vitro. In particular, the present invention provides methods for culturing the eyeball, including the retina, and the inner ear, including the Organ of Corti, in vitro. The methods of the present invention involve little or no microsurgical dissection of the entire fluid-filled sensory organ, thereby preserving the structural and functional integrity of the sensory epithelium. The methods of the present invention include the steps of (a) introducing a fluid-filled sensory organ (such as an inner ear or an eye ball) into a culture chamber containing liquid culture medium and (b) moving the culture chamber so that the fluid-filled sensory organ moves within the culture chamber. Preferably the culture chamber is cylindrical or annular in shape and is rotated about its longitudinal axis.

IPC 1-7

C12N 5/08; A01N 1/02; C12M 3/02; C12M 3/00; C12N 5/06

IPC 8 full level

A61K 38/17 (2006.01); C12N 5/06 (2006.01); C12N 5/0793 (2010.01); A61K 48/00 (2006.01)

CPC (source: EP)

A61K 38/1709 (2013.01); C12N 5/062 (2013.01); A61K 48/00 (2013.01); C12N 2501/40 (2013.01); C12N 2501/60 (2013.01)

Citation (search report)

  • [DA] WO 9507344 A1 19950316 - SCHWARZ RAY P [US], et al
  • [A] M. ANNIKO: "Antibiotics and sensorineural interactions.", ACTA OTOLARYNGOL. (STOCKH), vol. S429, 1986, pages 17 - 21, XP002222356
  • [A] CH.-P. LING & M. BOEHNKE: "iNFLUENCES OF METHYLCELLULOSE ON CORNEAL EPITHELIAL WOUND HEALING.", J. OCULAR PHARMACOL. THERAPEUT., vol. 15, no. 1, 1999, pages 59 - 63, XP001119933
  • See references of WO 0054583A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0054583 A1 20000921; AU 3616400 A 20001004; CA 2364024 A1 20000921; EP 1161142 A1 20011212; EP 1161142 A4 20030122

DOCDB simple family (application)

US 0005736 W 20000303; AU 3616400 A 20000303; CA 2364024 A 20000303; EP 00914825 A 20000303