Global Patent Index - EP 1161529 A2

EP 1161529 A2 2001-12-12 - END SELECTION IN DIRECTED EVOLUTION

Title (en)

END SELECTION IN DIRECTED EVOLUTION

Title (de)

SELEKTION AM ENDE IN GEZIELTER EVOLUTION

Title (fr)

SELECTION FINALE D'UNE EVOLUTION DIRIGEE

Publication

EP 1161529 A2 (EN)

Application

EP 00917887 A

Priority

  • US 0006497 W
  • US 26711899 A
  • US 27686099 A
  • US 33283599 A

Abstract (en)

[origin: WO0053744A2] This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of end-selection-based methods is the ability to recover full-length polynucleotides from a library of progeny molecules generated by mutagenesis methods. These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis™) and non-stochastic polynucleotide reassembly (GeneReassembly™). This invention provides methods of obtaining novel enzymes that have optmized physical and/or biological properties. Through use of the claimed methods, genetic vaccines, enzymes, small molecules, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression, increased uptake into a cell, increased stability in a cell, ability to tailor an immune response, and the like. Furthermore, this invention provides methods of obtaining a variety of novel biologically active molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.

IPC 1-7 (main, further and additional classification)

C12N 15/10; C12N 9/14; C12N 15/55; C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 9/14 (2006.01); C12N 15/10 (2006.01); C40B 40/02 (2006.01); C40B 50/06 (2006.01)

CPC (invention and additional information)

C12N 15/1058 (2013.01); C12N 9/14 (2013.01); C12N 15/102 (2013.01); C12N 15/1027 (2013.01); C12N 15/1034 (2013.01)

Citation (search report)

See references of WO 0053744A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0053744 A2 20000914; WO 0053744 A3 20010118; WO 0053744 A9 20010628; AU 3879300 A 20000928; CA 2361927 A1 20000914; EP 1161529 A2 20011212; IL 145165 D0 20020630; JP 2002537836 A 20021112

INPADOC legal status


2007-10-03 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20070411

2007-01-03 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20041206

2005-01-19 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20041206

2001-12-12 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20011009

2001-12-12 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-12-12 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL;LT;LV;MK;RO;SI