EP 1161529 A2 20011212 - END SELECTION IN DIRECTED EVOLUTION
Title (en)
END SELECTION IN DIRECTED EVOLUTION
Title (de)
SELEKTION AM ENDE IN GEZIELTER EVOLUTION
Title (fr)
SELECTION FINALE D'UNE EVOLUTION DIRIGEE
Publication
Application
Priority
- US 0006497 W 20000309
- US 26711899 A 19990309
- US 27686099 A 19990326
- US 33283599 A 19990614
Abstract (en)
[origin: WO0053744A2] This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of end-selection-based methods is the ability to recover full-length polynucleotides from a library of progeny molecules generated by mutagenesis methods. These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis™) and non-stochastic polynucleotide reassembly (GeneReassembly™). This invention provides methods of obtaining novel enzymes that have optmized physical and/or biological properties. Through use of the claimed methods, genetic vaccines, enzymes, small molecules, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression, increased uptake into a cell, increased stability in a cell, ability to tailor an immune response, and the like. Furthermore, this invention provides methods of obtaining a variety of novel biologically active molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.
IPC 1-7
IPC 8 full level
C12N 9/14 (2006.01); C12N 15/10 (2006.01); C40B 40/02 (2006.01); C40B 50/06 (2006.01)
CPC (source: EP)
C12N 9/14 (2013.01); C12N 15/102 (2013.01); C12N 15/1027 (2013.01); C12N 15/1034 (2013.01); C12N 15/1058 (2013.01)
Citation (search report)
See references of WO 0053744A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0053744 A2 20000914; WO 0053744 A3 20010118; WO 0053744 A9 20010628; AU 3879300 A 20000928; CA 2361927 A1 20000914; EP 1161529 A2 20011212; IL 145165 A0 20020630; JP 2002537836 A 20021112
DOCDB simple family (application)
US 0006497 W 20000309; AU 3879300 A 20000309; CA 2361927 A 20000309; EP 00917887 A 20000309; IL 14516500 A 20000309; JP 2000603365 A 20000309