Global Patent Index - EP 1161544 A1

EP 1161544 A1 2001-12-12 - GENERATION OF CONDITIONAL YEAST MUTANTS, METHODS AND REAGENTS RELATED THERETO

Title (en)

GENERATION OF CONDITIONAL YEAST MUTANTS, METHODS AND REAGENTS RELATED THERETO

Title (de)

ERZEUGUNG VON KONDITIONALE HEFEMUTANTEN, VERFAHREN UND REAGENZIEN DAZU

Title (fr)

GENERATION DE MUTANTS DE LEVURE CONDITIONNELS, TECHNIQUES ET REACTIFS S'Y RAPPORTANT

Publication

EP 1161544 A1 (EN)

Application

EP 00913902 A

Priority

  • US 0006514 W
  • US 12380799 P

Abstract (en)

[origin: WO0053781A1] The invention features a method for systematically generating mutant strains of obligate haploid and obligate diploid yeast, having condition-sensitive lethal mutations of a gene which is otherwise essential to cell viability under all conditions of cell growth. Conditional mutants make possible the analysis of physiological changes caused by inactivation of a gene or a gene product and can be used to address the function of any gene. In an exemplary embodiment, the method of the present invention can be generally characterized as follows: (i) providing a Candida strain whichis heterozygous for a loss-of-function mutation of an allele of an essential gene; and (ii) introducing a conditionally sensitive homolog of the gene, e.g., with conditional-sensitive protein or conditional-sensitive transcriptional regulatory sequence, at the functional allele by integrative transformation.

IPC 1-7 (main, further and additional classification)

C12N 15/81; C12N 1/19; C12N 15/31; C12N 15/90; C12Q 1/00

IPC 8 full level (invention and additional information)

C12N 1/19 (2006.01); C12N 15/31 (2006.01); C12N 15/81 (2006.01); C12N 15/90 (2006.01)

CPC (invention and additional information)

C12N 15/815 (2013.01); C12N 15/905 (2013.01)

Citation (search report)

See references of WO 0053781A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0053781 A1 20000914; AU 3526200 A 20000928; CA 2366069 A1 20000914; EP 1161544 A1 20011212

INPADOC legal status


2003-01-08 [18W] APPLICATION WITHDRAWN

- Date of withdrawal: 20021112

2001-12-12 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20011010

2001-12-12 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-12-12 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI