EP 1161544 A1 20011212 - GENERATION OF CONDITIONAL YEAST MUTANTS, METHODS AND REAGENTS RELATED THERETO
Title (en)
GENERATION OF CONDITIONAL YEAST MUTANTS, METHODS AND REAGENTS RELATED THERETO
Title (de)
ERZEUGUNG VON KONDITIONALE HEFEMUTANTEN, VERFAHREN UND REAGENZIEN DAZU
Title (fr)
GENERATION DE MUTANTS DE LEVURE CONDITIONNELS, TECHNIQUES ET REACTIFS S'Y RAPPORTANT
Publication
Application
Priority
- US 0006514 W 20000313
- US 12380799 P 19990311
Abstract (en)
[origin: WO0053781A1] The invention features a method for systematically generating mutant strains of obligate haploid and obligate diploid yeast, having condition-sensitive lethal mutations of a gene which is otherwise essential to cell viability under all conditions of cell growth. Conditional mutants make possible the analysis of physiological changes caused by inactivation of a gene or a gene product and can be used to address the function of any gene. In an exemplary embodiment, the method of the present invention can be generally characterized as follows: (i) providing a Candida strain whichis heterozygous for a loss-of-function mutation of an allele of an essential gene; and (ii) introducing a conditionally sensitive homolog of the gene, e.g., with conditional-sensitive protein or conditional-sensitive transcriptional regulatory sequence, at the functional allele by integrative transformation.
IPC 1-7
IPC 8 full level
C12N 1/19 (2006.01); C12N 15/31 (2006.01); C12N 15/81 (2006.01); C12N 15/90 (2006.01)
CPC (source: EP)
C12N 15/815 (2013.01); C12N 15/905 (2013.01)
Citation (search report)
See references of WO 0053781A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0053781 A1 20000914; AU 3526200 A 20000928; CA 2366069 A1 20000914; EP 1161544 A1 20011212
DOCDB simple family (application)
US 0006514 W 20000313; AU 3526200 A 20000313; CA 2366069 A 20000313; EP 00913902 A 20000313