EP 1161551 A2 20011212 - METHODS AND MATERIALS FOR THE RAPID AND HIGH VOLUME PRODUCTION OF A GENE KNOCK-OUT LIBRARY IN AN ORGANISM
Title (en)
METHODS AND MATERIALS FOR THE RAPID AND HIGH VOLUME PRODUCTION OF A GENE KNOCK-OUT LIBRARY IN AN ORGANISM
Title (de)
METHODEN UND MATERIALIEN ZUR SCHNELLEN UND IN EINER GROSSEN AUSBEUTE RESULTIERENDEN HERSTELLUNG VON "KNOCK-OUT" GENBANKEN IN EINEM ORGANISMUS
Title (fr)
METHODES ET MATERIAUX DE PRODUCTION ELEVEE ET RAPIDE D'UNE BIBLIOTHEQUE DE GENES KNOCKOUTS DANS UN ORGANISME
Publication
Application
Priority
- US 0007317 W 20000316
- US 27062099 A 19990317
Abstract (en)
[origin: WO0055346A2] The present invention relates to a method for facilitating site directed homologous recombination in an organism to produce mutants comprising: 1) constructing a large insert vector library comprising one or more large insert vectors; 2) constructing a second vector comprising a transposable element; 3) incubating the product of step 1) with the product of step 2) in the presence of a transposase specific for the inverted repeat sequences on the plasmid vector; 4) amplifying the disrupted large insert vector resulting from step 3); 5) introducing the disrupted large insert vector produced by step 4) into a target host cell; and 6) selecting for successful homologous recombination produced by step 5) with the second selectable marker.
IPC 1-7
IPC 8 full level
C12N 9/22 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/90 (2006.01)
CPC (source: EP)
C12N 9/22 (2013.01); C12N 15/1034 (2013.01); C12N 15/90 (2013.01)
Citation (search report)
See references of WO 0055346A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0055346 A2 20000921; WO 0055346 A3 20010215; AU 3900700 A 20001004; EP 1161551 A2 20011212; JP 2003502015 A 20030121
DOCDB simple family (application)
US 0007317 W 20000316; AU 3900700 A 20000316; EP 00918144 A 20000316; JP 2000605763 A 20000316