Global Patent Index - EP 1161551 A2

EP 1161551 A2 2001-12-12 - METHODS AND MATERIALS FOR THE RAPID AND HIGH VOLUME PRODUCTION OF A GENE KNOCK-OUT LIBRARY IN AN ORGANISM

Title (en)

METHODS AND MATERIALS FOR THE RAPID AND HIGH VOLUME PRODUCTION OF A GENE KNOCK-OUT LIBRARY IN AN ORGANISM

Title (de)

METHODEN UND MATERIALIEN ZUR SCHNELLEN UND IN EINER GROSSEN AUSBEUTE RESULTIERENDEN HERSTELLUNG VON "KNOCK-OUT" GENBANKEN IN EINEM ORGANISMUS

Title (fr)

METHODES ET MATERIAUX DE PRODUCTION ELEVEE ET RAPIDE D'UNE BIBLIOTHEQUE DE GENES KNOCKOUTS DANS UN ORGANISME

Publication

EP 1161551 A2 (EN)

Application

EP 00918144 A

Priority

  • US 0007317 W
  • US 27062099 A

Abstract (en)

[origin: WO0055346A2] The present invention relates to a method for facilitating site directed homologous recombination in an organism to produce mutants comprising: 1) constructing a large insert vector library comprising one or more large insert vectors; 2) constructing a second vector comprising a transposable element; 3) incubating the product of step 1) with the product of step 2) in the presence of a transposase specific for the inverted repeat sequences on the plasmid vector; 4) amplifying the disrupted large insert vector resulting from step 3); 5) introducing the disrupted large insert vector produced by step 4) into a target host cell; and 6) selecting for successful homologous recombination produced by step 5) with the second selectable marker.

IPC 1-7 (main, further and additional classification)

C12N 15/90; C12N 9/22; C12N 15/10

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/90 (2006.01)

CPC (invention and additional information)

C12N 15/90 (2013.01); C12N 9/22 (2013.01); C12N 15/1034 (2013.01)

Citation (search report)

See references of WO 0055346A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0055346 A2 20000921; WO 0055346 A3 20010215; AU 3900700 A 20001004; EP 1161551 A2 20011212; JP 2003502015 A 20030121

INPADOC legal status


2006-04-19 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20051001

2005-03-02 [RAP1] TRANSFER OF RIGHTS OF AN APPLICATION

- Owner name: ICORIA, INC.

2001-12-12 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010913

2001-12-12 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-12-12 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI