Global Patent Index - EP 1161561 A1

EP 1161561 A1 2001-12-12 - ONE STEP SAMPLE PREPARATION AND DETECTION OF NUCLEIC ACIDS IN COMPLEX BIOLOGICAL SAMPLES

Title (en)

ONE STEP SAMPLE PREPARATION AND DETECTION OF NUCLEIC ACIDS IN COMPLEX BIOLOGICAL SAMPLES

Title (de)

EINSTUFIGE PROBEZUBEREITUNG UND BESTIMMUNG VON NUKLEINSÄURE IN BIOLOGISCHEN PROBEN

Title (fr)

PREPARATION D'ECHANTILLONS ET DETECTION D'ACIDES NUCLEIQUES EN UNE ETAPE DANS DES ECHANTILLONS BIOLOGIQUES COMPLEXES

Publication

EP 1161561 A1 (EN)

Application

EP 00910584 A

Priority

  • DK 0000128 W
  • DK PA199900384 A

Abstract (en)

[origin: WO0056920A1] A method for stimultaneous release and detection of nucleic acids from complex biological samples is described. The invention relates to the combined use of lysis buffers containing strong chaotropic agents such as guanidine thiocyanate to facilitate cell lysis and release of cellular nucleic acids and to the use of a novel type of bicyclic nucleotide analogues, locked nucleic acid (LNA) to detect specific nucleic acids released during lysis by nucleic acid hybridisation. In particular methods are described for the covalent attachment of the catching LNA-oligo. Novel methods for sample preparation of e.g. polyadenylated mRNA species are also presented. The invention further addresses reagents for performing the methods as well as reagents and applications of the method.

IPC 1-7 (main, further and additional classification)

C12Q 1/68; C07K 19/00

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C07K 14/00 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6816 (2013.01); C07K 14/003 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6837 (2013.01)

Combination set (CPC)

  1. C12Q 1/6806 + C12Q 2525/307 + C12Q 2527/125 + C12Q 2565/101
  2. C12Q 1/6806 + C12Q 2537/125 + C12Q 2537/101 + C12Q 2527/125 + C12Q 2527/101
  3. C12Q 1/6816 + C12Q 2525/307 + C12Q 2527/125 + C12Q 2565/101
  4. C12Q 1/6837 + C12Q 2525/307 + C12Q 2527/125
  5. C12Q 1/6837 + C12Q 2525/307 + C12Q 2527/125

Citation (search report)

See references of WO 0056920A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family

WO 0056920 A1 20000928; AT 314488 T 20060115; AU 3274600 A 20001009; CA 2366231 A1 20000928; CA 2366231 C 20100511; CN 1350591 A 20020522; DE 60025172 D1 20060202; DE 60025172 T2 20060921; EP 1161561 A1 20011212; EP 1161561 B1 20051228; IL 145422 D0 20020630; JP 2002539802 A 20021126; JP 4975905 B2 20120711; KR 20020007332 A 20020126; US 2003039974 A1 20030227; US 6303315 B1 20011016