EP 1161681 A2 20011212 - USE OF LNA IN MASS SPECTROMETRY
Title (en)
USE OF LNA IN MASS SPECTROMETRY
Title (de)
VERWENDUNG VON LNA IN MASSENSPEKTROMETRIE
Title (fr)
UTILISATION D'ANALOGUES NUCLEOSIDIQUES BLOQUES EN SPECTROMETRIE DE MASSE
Publication
Application
Priority
- DK 0000126 W 20000317
- DK PA199900381 A 19990318
Abstract (en)
[origin: WO0057180A2] The present invention relates to DNA and RNA diagnostics based on mass spectrometry, e.g. Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight (MALDI-TOF) mass spectrometry, Electrospray (ES) mass spectrometry, Ion Cyclotron Resonance (ICR) mass spectrometry, Fourier Transform mass spectrometry, or combinations thereof, where fully or partially LNA modified DNA probes are used in order to enhance stability and resolution. The invention in particular relates to a process for detecting a target nucleic acid sequence of a nucleic acid molecule or for detecting a mutation in a nucleic acid sequence of a nucleic acid molecule, wherein (a) the nucleic acid molecule or (b) a part of the nucleic acid molecule or (c) an oligonucleotide complementary to the sequence or at least a sub-sequence of the nucleic acid molecule is analysed by mass spectrometry in order to obtain direct or indirect information about said target nucleic acid sequence or mutation, and wherein the process involves the hybridisation of an LNA modified oligonucleotide to the nucleic acid molecule.
IPC 1-7
IPC 8 full level
C07H 19/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6827 (2018.01); G01N 27/62 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
C07H 19/00 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6827 (2013.01)
Citation (search report)
See references of WO 0057180A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0057180 A2 20000928; WO 0057180 A3 20010208; WO 0057180 A8 20010503; AU 3274400 A 20001009; EP 1161681 A2 20011212; IL 145421 A0 20020630; JP 2002540402 A 20021126
DOCDB simple family (application)
DK 0000126 W 20000317; AU 3274400 A 20000317; EP 00910582 A 20000317; IL 14542100 A 20000317; JP 2000607004 A 20000317