Global Patent Index - EP 1161681 A2

EP 1161681 A2 2001-12-12 - USE OF LNA IN MASS SPECTROMETRY

Title (en)

USE OF LNA IN MASS SPECTROMETRY

Title (de)

VERWENDUNG VON LNA IN MASSENSPEKTROMETRIE

Title (fr)

UTILISATION D'ANALOGUES NUCLEOSIDIQUES BLOQUES EN SPECTROMETRIE DE MASSE

Publication

EP 1161681 A2 (EN)

Application

EP 00910582 A

Priority

  • DK 0000126 W
  • DK PA199900381 A

Abstract (en)

[origin: WO0057180A2] The present invention relates to DNA and RNA diagnostics based on mass spectrometry, e.g. Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight (MALDI-TOF) mass spectrometry, Electrospray (ES) mass spectrometry, Ion Cyclotron Resonance (ICR) mass spectrometry, Fourier Transform mass spectrometry, or combinations thereof, where fully or partially LNA modified DNA probes are used in order to enhance stability and resolution. The invention in particular relates to a process for detecting a target nucleic acid sequence of a nucleic acid molecule or for detecting a mutation in a nucleic acid sequence of a nucleic acid molecule, wherein (a) the nucleic acid molecule or (b) a part of the nucleic acid molecule or (c) an oligonucleotide complementary to the sequence or at least a sub-sequence of the nucleic acid molecule is analysed by mass spectrometry in order to obtain direct or indirect information about said target nucleic acid sequence or mutation, and wherein the process involves the hybridisation of an LNA modified oligonucleotide to the nucleic acid molecule.

IPC 1-7 (main, further and additional classification)

G01N 33/483

IPC 8 full level (invention and additional information)

G01N 27/62 (2006.01); C07H 19/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)

CPC (invention and additional information)

C12Q 1/6816 (2013.01); C07H 19/00 (2013.01); C12Q 1/6827 (2013.01)

Combination set (CPC)

C12Q 1/6827 + C12Q 2565/627 + C12Q 2565/518 + C12Q 2525/101

Citation (search report)

See references of WO 0057180A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0057180 A2 20000928; WO 0057180 A3 20010208; WO 0057180 A8 20010503; AU 3274400 A 20001009; EP 1161681 A2 20011212; IL 145421 D0 20020630; JP 2002540402 A 20021126

INPADOC legal status


2003-10-15 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030415

2002-09-25 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20020812

2001-12-12 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010919

2001-12-12 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-12-12 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 20010919;LT PAYMENT 20010919;LV PAYMENT 20010919;MK PAYMENT 20010919;RO PAYMENT 20010919;SI PAYMENT 20010919