Global Patent Index - EP 1163330 A1

EP 1163330 A1 2001-12-19 - DETECTION, CLONING AND SEQUENCING OF POLYPEPTIDES WHICH DRIVE THE SUBCELLULAR LOCALIZATION OF PROTEINS

Title (en)

DETECTION, CLONING AND SEQUENCING OF POLYPEPTIDES WHICH DRIVE THE SUBCELLULAR LOCALIZATION OF PROTEINS

Title (de)

ERKENNUNG, KLONIERUNG UND SEQUENZIERUNG VON POLYPEPTIDEN, DIE DIE SUBZELLULÄRE LOKALISIERUNG VON PROTEINEN STEUERN

Title (fr)

DETECTION, CLONAGE ET SEQUENAGE DE POLYPEPTIDES QUI COMMANDENT LA LOCALISATION SUBCELLULAIRE DE PROTEINES

Publication

EP 1163330 A1 (EN)

Application

EP 00912640 A

Priority

  • EP 00912640 A
  • EP 0002607 W
  • EP 99105846 A
  • EP 99120622 A

Abstract (en)

[origin: WO0056875A1] The present invention concerns a process for the detection, cloning and/or sequencing of polypeptides or parts thereof, which drive the subcellular localization of a protein containing such polypeptide or part thereof, characterized in that the process comprises the following steps: (a) constructing an expression library of random nucleic acids ligated to a reporter gene and contained in a vector molecule, (b) transfecting a plurality of host cells with the library, (c) screening for the subcellular localization of the expression product of the nucleic acid in the host cells via detection of a signal produced by the reporter gene, (d) cloning such cells where the reporter gene signal is detected in a certain subcellular localization, and (e) cloning and optionally sequencing the nucleic acid insert which encodes the polypeptide or part thereof. Polypeptides, driving the intracellular localization can be used to construct fusion proteins with predetermined intracellular localization.

IPC 1-7 (main, further and additional classification)

C12N 15/10; C07K 14/435; C12N 15/12; C12N 15/62

IPC 8 full level (invention and additional information)

C07K 14/435 (2006.01); C12N 15/10 (2006.01); C12N 15/12 (2006.01); C12N 15/62 (2006.01)

CPC (invention and additional information)

C12N 15/1051 (2013.01); C12N 15/1086 (2013.01); C07K 2319/00 (2013.01); C07K 2319/60 (2013.01)

Citation (search report)

See references of WO 0056875A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0056875 A1 20000928; WO 0056875 A9 20020228; AU 3431700 A 20001009; EP 1163330 A1 20011219

INPADOC legal status


2005-03-30 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20040917

2003-08-13 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030626

2001-12-19 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010917

2001-12-19 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE