EP 1163345 A1 20011219 - FUNGAL BETA-TUBULIN GENES

Title (en)

FUNGAL BETA-TUBULIN GENES

Title (de)

FUNGUS BETA-TUBULIN GENEN

Title (fr)

GENES FONGIQUES DE BETA-TUBULINE

Publication

EP 1163345 A1 20011219 (EN)

Application

EP 00919658 A 20000323

Priority

US 0007995 W 20000323
US 12571799 P 19990323

Abstract (en)

[origin: WO0056894A1] The anticancer drug taxol binds to beta-tubulin in assembled microtubules (MT) and causes cell cycle arrest in animal cells; in contrast, the effect of taxol varies in fungi. For instance, the taxol-producer Pestalotiopsis microspora Ne32, an ascomycete, is resistant to taxol (1C50>11.7 M), whereas Pythium ultimum and Phytophthora cinnamomi, two oomycetes, are sensitive to taxol (IC50 0.1 mu M). cDNAs encoding beta-tubulin from P.microspora, P. ultimum, and P. cinnamomi were isolated. The deduced amino acid sequences of beta-tubulin from P. microspora, P.ultimum, and P. cinnamomi can be used in (1) binding assays for the detection of taxol and taxol-like substances; (2) diagnostic assays for the pharmacologic efficacy of taxol against a tumor sample; (3) designing drugs with taxol-like activity via application of information regarding the effect of specific residues on taxol binding; and (4) detection of taxol and taxol-like activity via use of taxol-sensitive and taxol-resistant isogenic strains of P.ultimum constructed by substitution of residues necessary for taxol binding.

IPC 1-7

C12N 15/31; C07K 14/37; C12Q 1/04

IPC 8 full level

A01N 43/20 (2006.01); A01N 63/00 (2006.01); A01N 63/04 (2006.01); A61K 45/00 (2006.01); A61P 31/10 (2006.01); A61P 35/00 (2006.01); C07K 14/37 (2006.01); C12N 1/13 (2006.01); C12N 15/09 (2006.01); C12N 15/31 (2006.01); C12Q 1/02 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/569 (2006.01)

CPC (source: EP)

A61P 31/10 (2017.12); A61P 35/00 (2017.12); C07K 14/37 (2013.01); G01N 33/56961 (2013.01)

Citation (search report)

See references of WO 0056894A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0056894 A1 20000928; AU 4031100 A 20001009; CA 2367471 A1 20000928; EP 1163345 A1 20011219; JP 2002541782 A 20021210

DOCDB simple family (application)

US 0007995 W 20000323; AU 4031100 A 20000323; CA 2367471 A 20000323; EP 00919658 A 20000323; JP 2000606753 A 20000323