Global Patent Index - EP 1163345 A1

EP 1163345 A1 2001-12-19 - FUNGAL BETA-TUBULIN GENES

Title (en)

FUNGAL BETA-TUBULIN GENES

Title (de)

FUNGUS BETA-TUBULIN GENEN

Title (fr)

GENES FONGIQUES DE BETA-TUBULINE

Publication

EP 1163345 A1 (EN)

Application

EP 00919658 A

Priority

  • US 0007995 W
  • US 12571799 P

Abstract (en)

[origin: WO0056894A1] The anticancer drug taxol binds to beta-tubulin in assembled microtubules (MT) and causes cell cycle arrest in animal cells; in contrast, the effect of taxol varies in fungi. For instance, the taxol-producer Pestalotiopsis microspora Ne32, an ascomycete, is resistant to taxol (1C50>11.7 M), whereas Pythium ultimum and Phytophthora cinnamomi, two oomycetes, are sensitive to taxol (IC50 0.1 mu M). cDNAs encoding beta-tubulin from P.microspora, P. ultimum, and P. cinnamomi were isolated. The deduced amino acid sequences of beta-tubulin from P. microspora, P.ultimum, and P. cinnamomi can be used in (1) binding assays for the detection of taxol and taxol-like substances; (2) diagnostic assays for the pharmacologic efficacy of taxol against a tumor sample; (3) designing drugs with taxol-like activity via application of information regarding the effect of specific residues on taxol binding; and (4) detection of taxol and taxol-like activity via use of taxol-sensitive and taxol-resistant isogenic strains of P.ultimum constructed by substitution of residues necessary for taxol binding.

IPC 1-7 (main, further and additional classification)

C12N 15/31; C07K 14/37; C12Q 1/04

IPC 8 full level (invention and additional information)

G01N 33/50 (2006.01); A01N 43/20 (2006.01); A01N 63/00 (2006.01); A01N 63/04 (2006.01); A61K 45/00 (2006.01); A61P 31/10 (2006.01); A61P 35/00 (2006.01); C07K 14/37 (2006.01); C12N 1/13 (2006.01); C12N 15/09 (2006.01); C12N 15/31 (2006.01); C12Q 1/02 (2006.01); G01N 33/15 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/569 (2006.01)

CPC (invention and additional information)

G01N 33/56961 (2013.01); C07K 14/37 (2013.01)

Citation (search report)

See references of WO 0056894A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0056894 A1 20000928; AU 4031100 A 20001009; CA 2367471 A1 20000928; EP 1163345 A1 20011219; JP 2002541782 A 20021210

INPADOC legal status


2004-04-07 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20031001

2001-12-19 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20011011

2001-12-19 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-12-19 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI