EP 1163367 A2 20011219 - ANALYSIS OF DIFFERENTIAL GENE EXPRESSION
Title (en)
ANALYSIS OF DIFFERENTIAL GENE EXPRESSION
Title (de)
ANALYSE VON UNTERSCHIEDEN IN DER GENEXPRESSION
Title (fr)
ANALYSE DE L'EXPRESSION GENIQUE DIFFERENTIELLE
Publication
Application
Priority
- GB 0000807 W 20000309
- GB 9905807 A 19990312
Abstract (en)
[origin: WO0055363A2] The invention provides methods for detecting the differential expression or presence of two analytes, and more specifically to procedures which provide for rapid and efficient analysis of gene expression in biological systems. In particular, the invention provides a method of detecting and analysing differences between nucleic acids from two sources, which method comprises: a. providing nucleic acids from two sources as labelled probes; b. forming a mixture of the labelled probes with pooled reagents wherein each reagent is a population of beads carrying a polynucleotide target, the target of one reagent being different from the target of another reagent, the beads of one reagent being distinguishable from the beads of another reagent; c. incubating the mixture under conditions to promote specific hybridisation between probes and targets; and, d. analysing beads in the mixture by flow cytometry.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6809 (2018.01); C12Q 1/6834 (2018.01); G01N 15/14 (2006.01); G01N 21/78 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/58 (2006.01)
CPC (source: EP)
C12Q 1/6809 (2013.01); C12Q 1/6834 (2013.01); B01J 2219/005 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00664 (2013.01)
Citation (search report)
See references of WO 0055363A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0055363 A2 20000921; WO 0055363 A3 20001221; AU 2928400 A 20001004; EP 1163367 A2 20011219; GB 9905807 D0 19990505; JP 2002538836 A 20021119
DOCDB simple family (application)
GB 0000807 W 20000309; AU 2928400 A 20000309; EP 00907817 A 20000309; GB 9905807 A 19990312; JP 2000605779 A 20000309