Global Patent Index - EP 1163367 A2

EP 1163367 A2 2001-12-19 - ANALYSIS OF DIFFERENTIAL GENE EXPRESSION

Title (en)

ANALYSIS OF DIFFERENTIAL GENE EXPRESSION

Title (de)

ANALYSE VON UNTERSCHIEDEN IN DER GENEXPRESSION

Title (fr)

ANALYSE DE L'EXPRESSION GENIQUE DIFFERENTIELLE

Publication

EP 1163367 A2 (EN)

Application

EP 00907817 A

Priority

  • GB 0000807 W
  • GB 9905807 A

Abstract (en)

[origin: WO0055363A2] The invention provides methods for detecting the differential expression or presence of two analytes, and more specifically to procedures which provide for rapid and efficient analysis of gene expression in biological systems. In particular, the invention provides a method of detecting and analysing differences between nucleic acids from two sources, which method comprises: a. providing nucleic acids from two sources as labelled probes; b. forming a mixture of the labelled probes with pooled reagents wherein each reagent is a population of beads carrying a polynucleotide target, the target of one reagent being different from the target of another reagent, the beads of one reagent being distinguishable from the beads of another reagent; c. incubating the mixture under conditions to promote specific hybridisation between probes and targets; and, d. analysing beads in the mixture by flow cytometry.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

G01N 33/53 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 15/14 (2006.01); G01N 21/78 (2006.01); G01N 33/566 (2006.01); G01N 33/58 (2006.01)

CPC (invention and additional information)

C12Q 1/6809 (2013.01); C12Q 1/6834 (2013.01); B01J 2219/005 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00664 (2013.01)

Combination set (CPC)

  1. C12Q 1/6809 + C12Q 2565/626 + C12Q 2565/102
  2. C12Q 1/6834 + C12Q 2565/626 + C12Q 2565/102

Citation (search report)

See references of WO 0055363A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family

WO 0055363 A2 20000921; WO 0055363 A3 20001221; AU 2928400 A 20001004; EP 1163367 A2 20011219; GB 9905807 D0 19990505; JP 2002538836 A 20021119