Title (en)

ZYMOGEN ACTIVATION SYSTEM

Title (de)

AKTIVIERUNGSSYSTEM FüR ZYMOGEN

Title (fr)

SYSTEME D'ACTIVATION DU ZYMOGENE

Publication

EP 1173590 A4 20030115 (EN)

Application

EP 00922167 A 20000413

Priority

• US 0009973 W 20000413
• US 30316299 A 19990430

Abstract (en)

[origin: WO0066709A2] We describe the DNA sequences encoding an expression vector system that will permit, through limited proteolysis, the activation of expressed zymogen precursor of (S1) serine proteases in a highly controlled and reproducible fashion. The processed expressed protein, once activated, is rendered in a form amenable to measuring the catalytic activity. This catalytic activity of the activated form, is often a more accurate representation of the mature S1 protease gene product relative to the unprocessed zymogen precursor. Thus, this series of zymogen activation constructs represents a significant system for the analysis and characterization of serine protease gene products.

IPC 1-7

C12N 15/62; C12N 15/57; C12N 15/63; C12N 15/79

IPC 8 full level

A61K 38/48 (2006.01); A61K 47/48 (2006.01); A61P 43/00 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/64 (2006.01); C12N 15/09 (2006.01); C12N 15/57 (2006.01); C12N 15/62 (2006.01); C12P 21/06 (2006.01); C12Q 1/37 (2006.01)

CPC (source: EP)

A61P 43/00 (2017.12); C12N 9/6424 (2013.01); C12N 15/62 (2013.01); C12P 21/06 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/04 (2013.01); C07K 2319/21 (2013.01); C07K 2319/42 (2013.01); C07K 2319/50 (2013.01)

Citation (search report)

• [X] US 5342762 A 19940830 - MOSHER DEANE F [US], et al
• [X] US 5726038 A 19980310 - CHRISTIANSEN LARS [DK], et al
• [X] US 5504001 A 19960402 - FOSTER DONALD C [US]
• See references of WO 0066709A2

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0066709 A2 20001109; WO 0066709 A3 20010215; WO 0066709 A9 20020829; AR 023818 A1 20020904; AU 4239600 A 20001117; CA 2372907 A1 20001109; EP 1173590 A2 20020123; EP 1173590 A4 20030115; JP 2003531567 A 20031028

DOCDB simple family (application)

US 0009973 W 20000413; AR P000102081 A 20000428; AU 4239600 A 20000413; CA 2372907 A 20000413; EP 00922167 A 20000413; JP 2000615734 A 20000413