Global Patent Index - EP 1175430 A4

EP 1175430 A4 20041222 - ANTISENSE-OLIGOS WITH BETTER STABILITY AND ANTISENSE EFFECT

Title (en)

ANTISENSE-OLIGOS WITH BETTER STABILITY AND ANTISENSE EFFECT

Title (de)

ANTISENSE-OLIGONUKLEOTIDE MIT VERBESSERTER STABILITÄT UND ANTISENSE-EFFEKT

Title (fr)

OLIGONUCLEOTIDES ANTISENS A STABILITE AMELIOREE ET A EFFET ANTISENS

Publication

EP 1175430 A4 20041222 (EN)

Application

EP 00913157 A 20000404

Priority

  • KR 0000305 W 20000404
  • KR 19990012297 A 19990408

Abstract (en)

[origin: WO0061595A1] The present invention relates to novel antisense (AS) oligos containing one or more antisense sequence to mRNA region with a less secondary structure. Particularly, the present invention relates to a covalently-closed multiple antisense (CMAS)-oligo, which is constructed to form a closed type by ligation using complementary primer, and a ribbon-type antisense (RiAS)-oligo, which is composed of two loops containing multiple antisense sequences and a stem connecting the two loops that is constructed to by ligation using complementary sequences at both 5 prime ends. Since the novel AS-oligos of this invention are extremely stable to exonuclease activities, and show a significant growth inhibition of tumor cells, pharmaceutical compositions containing the novel types of AS-oligos of the invention are effective for treatment cancer, immune diseases, infectious diseases, and other human diseases caused by aberrant gene expression.

IPC 1-7

C07H 21/00

IPC 8 full level

A61K 9/127 (2006.01); A61K 48/00 (2006.01); A61P 31/00 (2006.01); A61P 35/00 (2006.01); A61P 35/02 (2006.01); A61P 37/00 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12Q 1/68 (2006.01)

CPC (source: EP KR)

A61P 31/00 (2017.12 - EP); A61P 35/00 (2017.12 - EP); A61P 35/02 (2017.12 - EP); A61P 37/00 (2017.12 - EP); C07H 21/00 (2013.01 - KR); C12N 15/113 (2013.01 - EP); C12N 15/1135 (2013.01 - EP); C07K 2319/00 (2013.01 - EP); C12N 2310/111 (2013.01 - EP); C12N 2310/53 (2013.01 - EP)

Citation (search report)

  • [Y] US 5714320 A 19980203 - KOOL ERIC T [US]
  • [Y] US 5872105 A 19990216 - KOOL ERIC T [US]
  • [PX] ROWLEY PETER T ET AL: "Circular antisense oligonucleotides inhibit growth of chronic myeloid leukemia cells", MOLECULAR MEDICINE (NEW YORK), vol. 5, no. 10, October 1999 (1999-10-01), pages 693 - 700, XP009038838, ISSN: 1076-1551
  • [X] ROWLEY PETER T ET AL: "Inhibition of chronic myeloid leukemia cell growth by circular antisense oligonucleotides", JOURNAL OF CELLULAR BIOCHEMISTRY SUPPLEMENT, vol. 0, no. 18 PART A, 1994, & KEYSTONE SYMPOSIUM ON GENE THERAPY; COPPER MOUNTAIN, COLORADO, USA; JANUARY 15-22, 1994, pages 235, XP009038842, ISSN: 0733-1959
  • [X] ROWLEY P T ET AL: "Circular antisense oligonucleotides inhibit proliferation of chronic myeloid leukemia cells in a sequence-specific manner", BLOOD, vol. 82, no. 10 SUPPL. 1, 1993, & THIRTY-FIFTH ANNUAL MEETING OF THE AMERICAN SOCIETY OF HEMATOLOGY; ST. LOUIS, MISSOURI, USA; DECEMBER 3-7, 1993, pages 330A, XP009038841, ISSN: 0006-4971
  • [Y] ASHLEY G W ET AL: "Chemical synthesis of oligodeoxynucleotide dumbbells", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 30, no. 11, 1991, pages 2927 - 2933, XP002975791, ISSN: 0006-2960
  • [A] RUBIN E ET AL: "Convergent DNA synthesis: a non-enzymatic dimerization approach to circular oligodeoxynucleotides.", NUCLEIC ACIDS RESEARCH. 11 SEP 1995, vol. 23, no. 17, 11 September 1995 (1995-09-11), pages 3547 - 3553, XP002302640, ISSN: 0305-1048
  • See references of WO 0061595A1

Designated contracting state (EPC)

DE ES FR GB IT

DOCDB simple family (publication)

WO 0061595 A1 20001019; AU 3464000 A 20001114; AU 773641 B2 20040527; CA 2369976 A1 20001019; CN 100381455 C 20080416; CN 1346363 A 20020424; EP 1175430 A1 20020130; EP 1175430 A4 20041222; JP 2002540813 A 20021203; JP 3721308 B2 20051130; KR 100397274 B1 20030913; KR 20000065690 A 20001115; KR 20020013522 A 20020220; KR 20020013527 A 20020220

DOCDB simple family (application)

KR 0000305 W 20000404; AU 3464000 A 20000404; CA 2369976 A 20000404; CN 00805926 A 20000404; EP 00913157 A 20000404; JP 2000610864 A 20000404; KR 19990012297 A 19990408; KR 20017012845 A 20011008; KR 20017013303 A 20011008