EP 1180115 A1 20020220 - MINIMAL ISOLATION PEPTIDE SYNTHESIS PROCESS USING ION-EXCHANGE RESINS AS SCAVENGING AGENTS
Title (en)
MINIMAL ISOLATION PEPTIDE SYNTHESIS PROCESS USING ION-EXCHANGE RESINS AS SCAVENGING AGENTS
Title (de)
VERFAHREN ZUR HERSTELLUNG VON PEPTIDEN MIT MINIMALER ISOLIERUNG DURCH VERWENDUNG VON IONENAUSTAUSCH-RESINEN ALS EINFANG-AGENTEN
Title (fr)
PROCEDE DE SYNTHESE PEPTIDIQUE A ISOLEMENT MINIMAL FAISANT APPEL A DES RESINES ECHANGEUSES D'IONS COMME AGENTS DE PIEGEAGE
Publication
Application
Priority
- US 0014152 W 20000523
- US 32276299 A 19990526
- US 52889900 A 20000320
Abstract (en)
[origin: WO0071569A1] A process for the production of a polypeptide having a pre-determined number and sequence of amino acid residues, comprising the steps of first exposing a first substrate amino acid or peptide fragment to a stoichiometric excess of a second reactant amino acid or peptide fragment to form a condensation product; second, contacting the reaction solution from the first step with an insoluble scavenger to sequester the excess of the second reactant amino acid or peptide fragment; third, removing from the solution the sequestered excess second reactant amino acid or peptide fragment; fourth, subjecting the reaction solution to a reaction which removes the protecting group from either the N- or C-terminus of the condensation product of the first step; and fifth, if necessary, repeating the first through fourth steps. The method is capable of large-scale production of peptides in solution, is not subject to the one-terminus-only limitation of the solid-phase method, possesses the "cleanliness" of the solid-phase method and, like the solid-phase method, is capable of automation. Most importantly, however, the method of the present invention does not require the frequent isolation of intermediates in a lengthy synthetic sequence nor, necessarily, the removal of all contaminating by-products from the reaction mixture prior to subsequent processing steps.
IPC 1-7
IPC 8 full level
C07K 1/02 (2006.01); C07K 1/06 (2006.01); C07K 1/18 (2006.01); C07K 5/103 (2006.01); C07K 7/06 (2006.01); C40B 40/02 (2006.01); C40B 50/06 (2006.01)
CPC (source: EP)
C07K 1/02 (2013.01); Y02P 20/55 (2015.11)
Citation (search report)
See references of WO 0071569A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0071569 A1 20001130; CA 2373816 A1 20001130; EP 1180115 A1 20020220; JP 2003500415 A 20030107; MX PA01012082 A 20020722
DOCDB simple family (application)
US 0014152 W 20000523; CA 2373816 A 20000523; EP 00936209 A 20000523; JP 2000619824 A 20000523; MX PA01012082 A 20000523