Global Patent Index - EP 1182457 A1

EP 1182457 A1 20020227 - Method and reagent for the analysis of blood samples, in particular for veterinary applications

Title (en)

Method and reagent for the analysis of blood samples, in particular for veterinary applications

Title (de)

Verfahren und Reagens zur Analyse von Blutproben, im besonderen für tierärztliche Anwendungen

Title (fr)

Méthode et réactif pour l'analyse des échantillons sanguins, en particulier pour les applications vétérinaires

Publication

EP 1182457 A1 20020227 (EN)

Application

EP 00202889 A 20000817

Priority

EP 00202889 A 20000817

Abstract (en)

The invention relates to a lysing agent for use in the analysis of the blood and/or of a blood sample of a non-human mammal, said lysing agent comprising an aqueous solution, which is such that it provides, upon dilution to the final WBC/Hb dilution, a pH of between 4.0 and 7.5 for said final WBC/Hb dilution; and which contains at least one quaternary ammonium compound, in a concentration such that, upon dilution to the final WBC/Hb dilution, the total concentration of quaternary ammonium compounds in said final WBC/Hb dilution is between 1.5 and 4.5 grams per liter; and which optionally further contains one or more components for lysing agents known per se. The invention also relates to a diluent for use in the analysis of the blood and/or of a blood sample of a non-human mammal, said diluent comprising an aqueous solution: having an osmolality of between 290 and 400; having a pH of between 6.0 and 8.0; optionally at least one coagulant chosen from EDTA, citrate/citric acid and/or salicylic acid; or a combination thereof; and optionally further containing one or more components for diluents known per se. The lysing agent and diluent of the invention are suited for the analysis of blood or a blood sample of a non-human mammal - e.g. using an aperture impedance technique - and in particular for the analysis of blood or a blood sample from a cat, dog or horse.

IPC 1-7

G01N 33/50

IPC 8 full level

G01N 15/12 (2006.01); G01N 33/49 (2006.01); G01N 33/50 (2006.01)

CPC (source: EP US)

G01N 33/5094 (2013.01 - EP US)

Citation (search report)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

EP 1182457 A1 20020227; AT E384951 T1 20080215; AU 9243901 A 20020225; DE 60132598 D1 20080313; DE 60132598 T2 20090129; DK 1309862 T3 20080526; EP 1309862 A1 20030514; EP 1309862 B1 20080123; ES 2300362 T3 20080616; JP 2004506899 A 20040304; PT 1309862 E 20080605; US 2004014152 A1 20040122; WO 0214861 A1 20020221

DOCDB simple family (application)

EP 00202889 A 20000817; AT 01972799 T 20010817; AU 9243901 A 20010817; DE 60132598 T 20010817; DK 01972799 T 20010817; EP 01972799 A 20010817; ES 01972799 T 20010817; JP 2002519939 A 20010817; NL 0100611 W 20010817; PT 01972799 T 20010817; US 34391503 A 20030205