EP 1183535 A2 20020306 - HOMOGENEOUS TESTS FOR SEQUENTIALLY DETERMINING LIPOPROTEIN FRACTIONS
Title (en)
HOMOGENEOUS TESTS FOR SEQUENTIALLY DETERMINING LIPOPROTEIN FRACTIONS
Title (de)
HOMOGENES TESTVERFAHREN ZUR SEQUENTIELLEN BESTIMMUNG VON LIPOPROTEINFRAKTIONEN
Title (fr)
TESTS HOMOGENES PERMETTANT DE DETERMINER LES FRACTIONS DE LIPOPROTEINE DE MANIERE SEQUENTIELLE
Publication
Application
Priority
- US 0014827 W 20000526
- US 13670999 P 19990528
Abstract (en)
[origin: WO0073797A2] The invention provides new homogeneous assays for the determination of the amount of LDL-C, of HDL-C, and of total cholesterol present in a sample. The method comprises complexing a first lipoprotein fraction with a complex-forming agent, such as an antibody, using enzymes to detect cholesterol in the non-complexed lipoprotein fraction, measuring the amount of cholesterol in the non-complexed fraction to provide a first cholesterol value, and then dissociating the complexed lipoprotein fraction from the complex-forming agent so that that cholesterol is available to be a substrate for the enzymes. The total amount of cholesterol present in the sample can then be determined. Further, the first cholesterol value obtained can be subtracted from the total cholesterol to obtain a value for the first lipoprotein fraction present in the sample. Optionally, a triglyceride assay can then also be performed on the sample in the same tube.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); C12Q 1/60 (2006.01); C12Q 1/61 (2006.01); G01N 33/92 (2006.01)
CPC (source: EP)
C12Q 1/60 (2013.01); G01N 33/92 (2013.01); G01N 2800/044 (2013.01)
Citation (search report)
See references of WO 0073797A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0073797 A2 20001207; WO 0073797 A3 20010913; WO 0073797 A9 20020221; AU 5449300 A 20001218; AU 771951 B2 20040408; CA 2375210 A1 20001207; EP 1183535 A2 20020306; JP 2003501630 A 20030114
DOCDB simple family (application)
US 0014827 W 20000526; AU 5449300 A 20000526; CA 2375210 A 20000526; EP 00939404 A 20000526; JP 2001500866 A 20000526