EP 1188049 A1 20020320 - CAPILLARY ELECTROPHORESIS METHOD FOR SCREENING FOR AFFINITY LIGANDS USING A DETECTABLE COMPETITIVE LIGAND
Title (en)
CAPILLARY ELECTROPHORESIS METHOD FOR SCREENING FOR AFFINITY LIGANDS USING A DETECTABLE COMPETITIVE LIGAND
Title (de)
ELEKTROFORETISCHES KAPILLARVERFAHREN ZUR UNTERSUCHUNG VON AFFINITÄTSLIGANDEN MIT VERWENDUNG EINES NACHWEISBAREN KOMPETITIVEN LIGANDS
Title (fr)
PROCEDE D'ELECTROPHORESE CAPILLAIRE DESTINE AU CRIBLAGE DE LIGANDS D'AFFINITE UTILISANT UN LIGAND COMPETITIF DETECTABLE
Publication
Application
Priority
- US 0017490 W 20000623
- US 14071099 P 19990624
Abstract (en)
[origin: WO0079260A1] The invention relates to a capillary electrophoresis-based method of screening complex materials for any unidentified affinity ligand that binds to a target of interest. The method subjects a plug of a mixture of the target and a complex material sample, and a separate plug of a known, tight-binding competitive ligand, to capillary electrophoresis under conditions optimized to allow mingling of the two plugs during the capillary electrophoresis run. Preferably, migration of the competitive ligand is tracked.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 27/447 (2006.01); G01N 33/15 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
G01N 27/44726 (2013.01); G01N 27/447 (2013.01); G01N 2500/00 (2013.01)
Citation (search report)
See references of WO 0079260A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0079260 A1 20001228; CA 2375798 A1 20001228; EP 1188049 A1 20020320; JP 2003502665 A 20030121
DOCDB simple family (application)
US 0017490 W 20000623; CA 2375798 A 20000623; EP 00944872 A 20000623; JP 2001505179 A 20000623