EP 1188059 A2 20020320 - ENUMERATION METHOD OF ANALYTE DETECTION
Title (en)
ENUMERATION METHOD OF ANALYTE DETECTION
Title (de)
QUANTIFIZIERUNGSVERFAHREN ZUM NACHWEIS EINES ANALYTEN
Title (fr)
DETECTION D'ANALYTE SELON UN PROCEDE DE NUMERATION
Publication
Application
Priority
- US 9910917 W 19990513
- US 8525998 P 19980513
Abstract (en)
[origin: WO9958948A2] This invention is directed to an optically-based method and system for analyte detection using solid phase immobilization, specific analyte labels adapted for signal generation and corresponding processes for the utilization thereof. The enumeration detection method disclosed herein narrows the area for signal observation, thus, improving detectable signal to background ratio. The system is comprised of a platform/support for immobilizing a sample stage having a labeled sample (analyte complex) bound thereto, a radiation source, an optical apparatus for generating and directing radiation at said sample and a means for data collection and analysis. Upon engagement of the system, the sample generates a signal capable of differentiation from background signal, both of which are collected and imaged with a signal detector that generated a sample image to a data processing apparatus. Said apparatus receives signal measurements and, in turn, enumerates individual binding events. Generated signal may be increased via selected mass enhancement. The invention, enumeration assay methodology detecting individual binding events, may be used, for example, in analyses to detect analyte or confirm results in both research, commercial and point of care applications.
IPC 1-7
G01N 33/543; G01N 33/553; G01N 33/552; G01N 33/53; G01N 33/537; G01N 21/00; G01N 31/22; G01N 15/06; G01N 33/00; G01N 21/29; G01N 21/41; G01N 21/47; G01N 21/27; G01N 21/31; G01N 21/59; G01N 21/01; G01N 21/17; G01N 21/75; B01L 3/00; G01D 11/26; B01J 19/08
IPC 8 full level
G01N 33/483 (2006.01); G01N 21/21 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP)
B82Y 15/00 (2013.01); B82Y 30/00 (2013.01); G01N 33/54373 (2013.01)
Citation (search report)
See references of WO 9958948A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9958948 A2 19991118; WO 9958948 A3 20020103; AU 4190099 A 19991129; CA 2331787 A1 19991118; EP 1188059 A2 20020320; IL 139631 A0 20020210; JP 2002526743 A 20020820; NZ 508062 A 20020426
DOCDB simple family (application)
US 9910917 W 19990513; AU 4190099 A 19990513; CA 2331787 A 19990513; EP 99925655 A 19990513; IL 13963199 A 19990513; JP 2000548701 A 19990513; NZ 50806299 A 19990513