EP 1194591 A2 20020410 - METHOD AND KIT FOR DETERMINING DNA DOUBLE/SINGLE STRAND BREAKS AND DEVICE FOR CARRYING OUT THE CONTROLLED FILTRATION OF, IN PARTICULAR, DNA MOLECULES WITH WELL FILTER PLATES
Title (en)
METHOD AND KIT FOR DETERMINING DNA DOUBLE/SINGLE STRAND BREAKS AND DEVICE FOR CARRYING OUT THE CONTROLLED FILTRATION OF, IN PARTICULAR, DNA MOLECULES WITH WELL FILTER PLATES
Title (de)
VERFAHREN UND KIT ZUR BESTIMMUNG VON DNA-DOPPEL-/EINZELSTRANGBRÜCHEN UND VORRICHTUNG ZUM KONTROLLIERTEN FILTRIEREN VON INSBESONDERE DNA-MOLEKÜLEN MIT WELL-FILTERPLATTEN
Title (fr)
PROCEDE ET NECESSAIRE POUR LA DETERMINATION DE CASSURES BICATENAIRES OU MONOCATENAIRES D'ADN, ET DISPOSITIF POUR LA FILTRATION CONTROLEE EN PARTICULIER DE MOLECULES D'ADN AVEC DES PLAQUES DE FILTRATION A PUITS
Publication
Application
Priority
- DE 19933078 A 19990719
- EP 0006842 W 20000718
Abstract (en)
[origin: WO0106003A2] The invention relates to, among other things, a method for determining double strand breaks which comprises the following steps: a) depositing cells and/or tissues to be examined on specific filters; b) lysis of the cells/tissue with at least one detergent, with at least one chaotropic substance, and with at least one fluorescence dye that binds specifically a DNA double strand; c) elution of the DNA fragments, released by the lysis, through the specific filters, and; d) carrying out a fluorescence spectroscopic evaluation, whereby only one eluate fraction is collected per well.
IPC 1-7
IPC 8 full level
B01L 3/00 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6816 (2018.01)
CPC (source: EP)
B01L 3/50255 (2013.01); C12Q 1/6816 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0829 (2013.01); B01L 2400/049 (2013.01)
Citation (search report)
See references of WO 0106003A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL
DOCDB simple family (publication)
WO 0106003 A2 20010125; WO 0106003 A3 20011213; AU 6275100 A 20010205; DE 19933078 A1 20010208; DE 19933078 B4 20040708; EP 1194591 A2 20020410
DOCDB simple family (application)
EP 0006842 W 20000718; AU 6275100 A 20000718; DE 19933078 A 19990719; EP 00949369 A 20000718