EP 1198582 A1 20020424 - PREPARATION OF FREEZE/THAWED LIPID COMPLEXES
Title (en)
PREPARATION OF FREEZE/THAWED LIPID COMPLEXES
Title (de)
VORBEREITUNG VON EINGEFRORENE/AUFGETAUTE LIPIDKOMPLEXE
Title (fr)
PREPARATION DE COMPLEXES DE LIPIDE CONGELES/DECONGELES
Publication
Application
Priority
- GB 0003040 W 20000807
- GB 9918670 A 19990806
Abstract (en)
[origin: WO0111069A1] A process is described to enable the successful transfection of cells with lipid complexes in the presence of serum. The process involves subjecting a lipid complex to one or more freeze/thaw cycles and then bringing the freeze/thawed material into contact with cells either <i>ex vivo</i> or <i>in vivo</i>. The lipid complex may be any anionic, cationic or neutral lipid complexed with one or more other bioactive materials such as nucleic acids.
IPC 1-7
IPC 8 full level
C12N 15/88 (2006.01)
CPC (source: EP)
C12N 15/88 (2013.01)
Citation (search report)
See references of WO 0111069A1
Citation (examination)
- US 5914126 A 19990622 - LI LINGNA [US], et al
- STORM G.; CROMMELIN D.: "Liposomes: quo vadis?", PSTT, vol. 1, no. 1, 1 April 1998 (1998-04-01), pages 19 - 31
- MONNARD P. ET AL: "Entrapment of nucleic acids in liposomes", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1329, no. 1, 2 October 1997 (1997-10-02), AMSTERDAM, pages 39 - 50
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0111069 A1 20010215; AU 6306600 A 20010305; EP 1198582 A1 20020424; GB 9918670 D0 19991013
DOCDB simple family (application)
GB 0003040 W 20000807; AU 6306600 A 20000807; EP 00949803 A 20000807; GB 9918670 A 19990806