EP 1200625 A1 20020502 - METHODS FOR DETERMINING THE SPECIFICITY AND SENSITIVITY OF OLIGONUCLEOTIDES FOR HYBRIDIZATION
Title (en)
METHODS FOR DETERMINING THE SPECIFICITY AND SENSITIVITY OF OLIGONUCLEOTIDES FOR HYBRIDIZATION
Title (de)
VERFAHREN ZUR BESTIMMUNG DER SPEZIFITÄT UND SENSITIVITÄT VON OLIGONUKLEOTIDEN ZUR HYBRIDISIERUNG
Title (fr)
RECHERCHE DE SPECIFICITE ET DE SENSIBILITE A L'HYBRIDATION D'OLIGONUCLEOTIDES
Publication
Application
Priority
- US 0019203 W 20000714
- US 14438299 P 19990716
- US 15456399 P 19990917
Abstract (en)
[origin: WO0106013A1] The present invention provides materials and methods which may be used to evaluate one or more different probes and select probes that are optimized for sensitivity and specificity for a particular target. In particularly preferred embodiments, the methods and compositions of the invention can be used to evaluate polynucleotide probes having different nucleotide sequences. The methods and compositions thereby allow a user to select a polynucleotide probe, (e.g.), having a particular nucleotide sequence, that is optimized for sensitivity and/or for specificity for a particular target polynucleotide molecule. In particularly preferred embodiments, the methods and compositions can be used to evaluate a plurality of probes simultaneously, such as on a microarray. Probes evaluated according to the methods of the invention can be selected for and used to detect a variety of molecules, including a variety of polynucleotides, such as genomic polynucleotides (e.g., genomic DNA) and genomic transcripts (e.g.), mRNAs or cDNA sequences derived from there) as well as gene copy numbers of specific transcripts, single nucleotide polymorphisms, (etc.).
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 33/58 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP)
C12Q 1/6811 (2013.01)
C-Set (source: EP)
Citation (search report)
See references of WO 0106013A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0106013 A1 20010125; AU 6213600 A 20010205; CA 2379212 A1 20010125; EP 1200625 A1 20020502; JP 2003505038 A 20030212
DOCDB simple family (application)
US 0019203 W 20000714; AU 6213600 A 20000714; CA 2379212 A 20000714; EP 00948666 A 20000714; JP 2001511221 A 20000714