EP 1203076 A1 20020508 - METHOD FOR IN VITRO CULTURE OF VIRUSES OF THE TOGAVIRIDAE AND FLAVIVIRIDAE FAMILY AND USES

Title (en)

METHOD FOR IN VITRO CULTURE OF VIRUSES OF THE TOGAVIRIDAE AND FLAVIVIRIDAE FAMILY AND USES

Title (de)

VERFAHREN FÜR DIE IN VITRO ZÜCHTUNG VON VIREN DER FAMILIEN TOGAVIRIDAE UND FLAVIVIRIDAE UND IHRE ANWENDUNGEN

Title (fr)

PROCEDE DE CULTURE IN VITRO DE VIRUS DES FAMILLES TOGAVIRIDAE ET FLAVIVIRIDAE ET APPLICATIONS

Publication

EP 1203076 A1 20020508 (FR)

Application

EP 00956580 A 20000731

Priority

FR 0002202 W 20000731
FR 9910095 A 19990730

Abstract (en)

[origin: FR2796962A1] The invention concerns a method of culturing, propagating and replicating in vitro viruses of the Togaviridae and Flaviviridae families which consists in: providing at least a fraction of LVP's obtained from serum or plasma of a patient infected with at least a virus of the Togaviridae and Flaviviridae families; contacting said fraction, for a predetermined time interval in an appropriate culture medium, with permissive cells having an endocytosis path relayed by at least one receptor of the lipoproteins and modulated inter alia by an activating agent selected among an unsaturated fatty acid or an unsaturated fatty acid derivative comprising 16 to 20 carbon atoms or their mixture. The invention also concerns the uses of viral particles and polypeptides obtained by said method.

IPC 1-7

C12N 7/00; C12N 5/10; C12N 5/06

IPC 8 full level

A61K 39/12 (2006.01); A61P 31/14 (2006.01); C07K 16/08 (2006.01); C12N 5/02 (2006.01); C12N 7/00 (2006.01); C12P 21/08 (2006.01); C12Q 1/02 (2006.01); C12Q 1/70 (2006.01); G01N 33/569 (2006.01); C12R 1/93 (2006.01)

CPC (source: EP)

A61P 31/14 (2017.12); C12N 7/00 (2013.01); G01N 33/56983 (2013.01); C12N 2770/24251 (2013.01); G01N 2333/18 (2013.01)

Citation (search report)

See references of WO 0109289A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

FR 2796962 A1 20010202; FR 2796962 B1 20031121; AU 6847400 A 20010219; CA 2380407 A1 20010208; EP 1203076 A1 20020508; JP 2003506031 A 20030218; WO 0109289 A1 20010208

DOCDB simple family (application)

FR 9910095 A 19990730; AU 6847400 A 20000731; CA 2380407 A 20000731; EP 00956580 A 20000731; FR 0002202 W 20000731; JP 2001514083 A 20000731