EP 1204758 A2 20020515 - USE OF ASPERGILLUS SOJAE AS HOST FOR RECOMBINANT PROTEIN PRODUCTION
Title (en)
USE OF ASPERGILLUS SOJAE AS HOST FOR RECOMBINANT PROTEIN PRODUCTION
Title (de)
VERWENDUNG VON ASPERGILLUS SOJAE FÜR DIE HERSTELLUNG VON REKOMBINANTEN PROTEINEN
Title (fr)
UTILISATION D'ASPERGILLUS SOJAE COMME HOTE POUR LA PRODUCTION DE PROTEINES RECOMBINANTES
Publication
Application
Priority
- EP 00950088 A 20000728
- EP 99202516 A 19990730
- EP 99202517 A 19990730
- NL 0000544 W 20000728
Abstract (en)
[origin: WO0109352A2] A recombinant <i>Aspergillus sojae</i> comprising an introduced acetamidase S (<i>amdS</i>) gene as selectable marker is disclosed. An <i>Aspergillus sojae</i> exhibiting growth with medium comprising uracil and fluoro-orotic acid, said <i>Aspergillus sojae</i> further not exhibiting growth on medium comprising uridine and fluoro-orotic acid i.e. said <i>Aspergillus sojae</i> exhibiting uracil auxotrophy, said <i>Aspergillus sojae</i> being unable to utilize uridine, said <i>Aspergillus sojae</i> being pyrG negative, said <i>Aspergillus sojae</i> exhibiting resistance to fluoro-orotic acid, said uracil auxotrophy and said fluoro-orotic acid resistance being relievable upon complementation with an active introduced <i>pyrG</i> gene, is described. <i>The Aspergillus sojae</i> further comprises a nucleic acid sequence encoding a phytase or a protein having phytase activity or any other heterologous protein or polypeptide and can be used for the biotechnological production of said phytase or said other heterologous proteins or polypeptides. Additional mutants exhibiting amended morphology are also disclosed. Methods of producing such expression hosts are described.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 1/14 (2006.01); C12N 1/15 (2006.01); C12N 9/16 (2006.01); C12N 9/58 (2006.01); C12N 15/10 (2006.01); C12N 15/67 (2006.01); C12N 15/80 (2006.01); C12R 1/66 (2006.01)
CPC (source: EP KR)
C12N 1/14 (2013.01 - KR); C12N 9/58 (2013.01 - EP); C12N 15/80 (2013.01 - EP)
Citation (search report)
See references of WO 0109352A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0109352 A2 20010208; WO 0109352 A3 20011108; AR 024989 A1 20021106; AU 6323800 A 20010219; AU 782116 B2 20050707; BR 0012856 A 20020611; CA 2378016 A1 20010208; CN 1369016 A 20020911; EP 1204758 A2 20020515; JP 2003518921 A 20030617; KR 20020059350 A 20020712; MX PA02001061 A 20030410
DOCDB simple family (application)
NL 0000544 W 20000728; AR P000103931 A 20000728; AU 6323800 A 20000728; BR 0012856 A 20000728; CA 2378016 A 20000728; CN 00811088 A 20000728; EP 00950088 A 20000728; JP 2001514144 A 20000728; KR 20027001321 A 20020130; MX PA02001061 A 20000728