Global Patent Index - EP 1206477 A4

EP 1206477 A4 20041124 - CLONING OF THE STREPTOMYCES AVERMITILIS GENES FOR GLYCOSYLATION OF AVERMECTIN AGLYCONES

Title (en)

CLONING OF THE STREPTOMYCES AVERMITILIS GENES FOR GLYCOSYLATION OF AVERMECTIN AGLYCONES

Title (de)

KLONIERUNG DES STREPTOMYCES AVERMITILIS GENES ZUR GLYCOSYLIERUNG VON AVERMECTIN AGLYCONEN

Title (fr)

CLONAGE DES GENES STREPTOMYCES AVERMITILIS POUR LA GLYCOSYLATION D'AGLYCONES D'AVERMECTINE

Publication

EP 1206477 A4 20041124 (EN)

Application

EP 00950713 A 20000726

Priority

  • US 0020331 W 20000726
  • US 14669999 P 19990730

Abstract (en)

[origin: WO0109155A1] A cluster of genes involved in the synthesis and/or addition of oleandrose to avermectin aglycones has been cloned. A 11-kb <i>Pst</i>I clone complemented 28 avermectin glycosylation mutants in seven complementation classes. Sequencing of an 10-kb region identified 9 ORFs and an additional partial ORF. Eight of the ORFs were correlated to the seven glycosylation complementation classes. Sequence comparison to Genbank databases identified 6 genes: dTDP-glucose synthase; dTDP-glucose 4,6 dehydrase; dTDP-4-keto-hexose reductase; dTDP-hexose 3,5 epimerase; dTDP-hexose 3' O-methylase; and an avermectin aglycone-dTDP-oleandrose glycosyltransferase. The ninth ORF was essential for biosynthesis of the avermectin aglycones. The partial ORF encoded part of an avermectin polyketide synthase module 7.

IPC 1-7

C12N 9/00; C12N 15/52

IPC 8 full level

C12N 15/09 (2006.01); A61K 31/7048 (2006.01); A61P 33/10 (2006.01); C07K 14/36 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/52 (2006.01); C12P 21/02 (2006.01); C12R 1/465 (2006.01)

CPC (source: EP)

A61P 33/10 (2017.12); C12N 15/52 (2013.01)

Citation (search report)

  • [X] EP 0391594 A2 19901010 - MERCK & CO INC [US]
  • [X] MACNEIL D J ET AL: "ANALYSIS OF STREPTOMYCES AVERMITILIS GENES REQUIRED FOR AVERMECTIN BIOSYNTHESIS UTILIZING A NOVEL INTEGRATION VECTOR", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 111, no. 1, 1992, pages 61 - 68, XP001180832, ISSN: 0378-1119
  • [X] OCCI J L ET AL: "CLONING AND SEQUENCING A 5.2 KB REGION FROM STREPTOMYCES-AVERMITILIS INVOLVED IN GLYCOSYLATION OF AVERMECTIN", ABSTRACTS OF THE ANNUAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 90, 1990, 90TH ANNUAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY 1990, ANAHEIM, CALIFORNIA, USA, MAY 13-, pages 268, XP008036043, ISSN: 0094-8519
  • [X] DATABASE EMBL 10 February 1999 (1999-02-10), "Streptomyces cyanogenus landomycin biosynthetic gene cluster, complete sequence", XP002298610, Database accession no. AF080235
  • [A] SCHULMAN M D ET AL: "PURIFICATION AND IDENTIFICATION OF D-TDP-OLEANDROSE THE PRECURSOR OF THE OLEANDROSE UNITS OF THE AVERMECTINS", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 28, 1990, pages 16965 - 16970, XP002298197, ISSN: 0021-9258
  • See references of WO 0109155A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0109155 A1 20010208; CA 2380639 A1 20010208; EP 1206477 A1 20020522; EP 1206477 A4 20041124; JP 2003506022 A 20030218

DOCDB simple family (application)

US 0020331 W 20000726; CA 2380639 A 20000726; EP 00950713 A 20000726; JP 2001513962 A 20000726