EP 1206524 A2 20020522 - METHODS FOR GENERATING DOUBLED HAPLOID PLANTS
Title (en)
METHODS FOR GENERATING DOUBLED HAPLOID PLANTS
Title (de)
VERFAHREN ZUR HERSTELLUNG VON DOPPELT HAPLOIDEN PFLANZEN
Title (fr)
PROCEDES DE GENERATION DE PLANTES HAPLOIDES DOUBLEES
Publication
Application
Priority
- US 0018790 W 20000823
- US 15076199 P 19990826
- US 9919498 W 19990826
- US 38358899 A 19990826
Abstract (en)
[origin: WO0114518A2] The present invention provides methods for generating doubled haploid and/or haploid plants from microspores. The methods of the invention include the steps of (a) purifying microspores at a developmental stage amenable to androgenic induction; (b) subjecting said microspores to nutrient stress to obtain stressed microspores; (c) contacting said microspores with an amount of a sporophytic development inducer effective to induce sporophytic development, said contacting step occurring before, during, after, or overlapping with any portion of said nutrient stress step; and (d) culturing said isolated microspores with at least one live plant ovary or with an aliquot of plant ovary conditioned medium. The methods of the present invention can also include the step of genetically transforming the microspores to produce genetically transformed plants.
IPC 1-7
C12N 5/04; C12N 15/05; C12N 15/82; A01H 1/08; A01H 4/00; A01H 5/00
IPC 8 full level
A01H 4/00 (2006.01)
CPC (source: EP US)
A01H 1/08 (2013.01 - EP US); A01H 4/005 (2013.01 - EP); A01H 4/008 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0114518 A2 20010301; WO 0114518 A3 20011018; AU 7051800 A 20010319; EP 1206524 A2 20020522; EP 1206524 A4 20040929
DOCDB simple family (application)
US 0018790 W 20000823; AU 7051800 A 20000823; EP 00959147 A 20000823