EP 1209224 B1 20080507 - Detection of one or more target analytes in a sample using spatially localized analyte reproduction
Title (en)
Detection of one or more target analytes in a sample using spatially localized analyte reproduction
Title (de)
Nachweis von Ein oder Mehr Zielanalyten in einer Probe unter Verwendung von raumlicher lokalisierter Analytreproduktion
Title (fr)
Détection d'un ou plusieurs analytes cibles dans un échantillon utilisant la reproduction d'analytes localisés en espace
Publication
Application
Priority
US 72882600 A 20001128
Abstract (en)
[origin: EP1209224A2] The presence or absence of one or more target microbial analytes in a substance, such as a biological or environmental substance, is assayed by inoculating a growth medium with a sample of the substance. The medium may be combined with a labeled analyte-specific material (LASM) which can migrate through the substrate and which is homogeneously distributed throughout the medium. The LASM may be premixed with the medium, or may be added to the medium after inoculation with the substance. The nature of the medium is such that it will support target analyte reproduction so as to form target analyte colonies in or on the medium, and it will not allow the target analyte colonies to migrate on or within the medium. After the sample to be assayed is added to the medium, growth of the target analyte colonies in the sample will bind increasing quantities of the LASM, thereby creating localized intensely labeled areas in the medium which can be visually or photometrically detected. As the target analyte colonies grow on or in the medium they attract increasing quantities of the LASM which diffuses to the colonies through the medium causing the local target analyte colonies to become intensely labeled, which renders the colonies readily detectable. Each of the intensely labeled target analyte colonies in the medium may also be surrounded by areas of lower intensity due to local depletion of the LASM in the regions of colony growth . The number of intensely labeled target analyte colonies in the medium will be proportional to the concentration of the target analyte present in the sample. In the event that the target analyte is absent from the sample, then there will be no localized labeled microbe colonies in the medium. <IMAGE>
IPC 8 full level
C12M 1/34 (2006.01); G01N 33/559 (2006.01); C12Q 1/04 (2006.01); G01N 33/569 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP US)
G01N 33/569 (2013.01 - EP US); Y02A 50/30 (2018.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
EP 1209224 A2 20020529; EP 1209224 A3 20031203; EP 1209224 B1 20080507; AT E394471 T1 20080515; AU 777638 B2 20041028; AU 8933501 A 20020530; CA 2364148 A1 20020528; CA 2364148 C 20080916; CN 1306270 C 20070321; CN 1356549 A 20020703; DE 60133869 D1 20080619; JP 2002223745 A 20020813; JP 3984035 B2 20070926; NO 20015770 D0 20011127; NO 20015770 L 20020529; SG 95684 A1 20030423; US 6518038 B1 20030211
DOCDB simple family (application)
EP 01128139 A 20011127; AT 01128139 T 20011127; AU 8933501 A 20011108; CA 2364148 A 20011127; CN 01142438 A 20011128; DE 60133869 T 20011127; JP 2001360249 A 20011127; NO 20015770 A 20011127; SG 200106927 A 20011109; US 72882600 A 20001128