Global Patent Index - EP 1210450 A4

EP 1210450 A4 20030723 - METHODS AND TARGETS OF ANTIBIOTIC RESISTANCE

Title (en)

METHODS AND TARGETS OF ANTIBIOTIC RESISTANCE

Title (de)

VERFAHREN UND ZIELE VON ANTIBIOTISCHER RESISTENZ

Title (fr)

PROCEDES ET CIBLES DE RESISTANCE A UN ANTIBIOTIQUE

Publication

EP 1210450 A4 20030723 (EN)

Application

EP 00968994 A 20000818

Priority

  • US 0040676 W 20000818
  • US 14976199 P 19990819

Abstract (en)

[origin: WO0112842A1] The present invention provides for a method of evolving and selecting cells resistant to a selective agent by inducing directed evolution in continuous culture while applying both a mutagenic and selective agent to the cells to determine the cells having resistance. This also provides a method of generating mutant drug targets useful for screening for effective compounds. The Figure is ageneralized schematic diagram of a chemostat of the present invention.

IPC 1-7

C12N 15/00; C12N 1/38; C12N 15/10; C12Q 1/02

IPC 8 full level

C12Q 1/18 (2006.01)

CPC (source: EP)

C12Q 1/18 (2013.01)

Citation (search report)

  • [X] US 5652098 A 19970729 - FREYER JAMES P [US]
  • [A] US 4528773 A 19850716 - HICKOK LESLIE G [US]
  • [X] FERRERO LUCY ET AL: "Analysis of gyrA and grlA Mutations in Stepwise-Selected Ciprofloxacin-Resistant Mutants of Staphylococcus aureus.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 39, no. 7, 1995, pages 1554 - 1558, XP002241880, ISSN: 0066-4804
  • [X] WIEBE M G ET AL: "USE OF A SERIES OF CHEMOSTAT CULTURES TO ISOLATE 'IMPROVED' VARIANTS OF THE QUORN MYCO-PROTEIN FUNGUS, FUSARIUM GRAMINEARUM A3/5", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 140, no. PART 11, 1 November 1994 (1994-11-01), pages 3015 - 3021, XP000197157, ISSN: 1350-0872
  • [A] KOLTERMANN ANDRE ET AL: "Principles and methods of evolutionary biotechnology.", BIOPHYSICAL CHEMISTRY, vol. 66, no. 2-3, 1997, pages 159 - 177, XP002241881, ISSN: 0301-4622
  • [A] BERTINO J R ET AL: "DRUG RESISTANT CELLS AS TARGETS FOR NEW THERAPIES", UCLA SYMPOSIA ON MOLECULAR AND CELLULAR BIOLOGY, NEW YORK, NY, US, 1985, pages 529 - 536, XP000568928, ISSN: 0735-9543
  • [DA] DYKHUIZEN D E: "CHEMOSTATS USED FOR STUDYING NATURAL SELECTION AND ADAPTIVE EVOLUTION", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 224, 1993, pages 613 - 631, XP001036679, ISSN: 0076-6879
  • See references of WO 0112842A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0112842 A1 20010222; WO 0112842 A9 20020801; AU 7882800 A 20010313; EP 1210450 A1 20020605; EP 1210450 A4 20030723

DOCDB simple family (application)

US 0040676 W 20000818; AU 7882800 A 20000818; EP 00968994 A 20000818