Global Patent Index - EP 1210451 A4

EP 1210451 A4 20040825 - RAPID METHOD FOR MEASURING COMPLEX CARBOHYDRATES IN MAMMALIAN TISSUE

Title (en)

RAPID METHOD FOR MEASURING COMPLEX CARBOHYDRATES IN MAMMALIAN TISSUE

Title (de)

SCHNELLVERFAHREN ZUM MESSEN KOMPLEXER KOHLENHYDRATE IN SÄUGERGEWEBEN

Title (fr)

METHODE RAPIDE DESTINEE A MESURER LA CONCENTRATION DE GLUCIDES COMPLEXES DANS LE TISSU MAMMALIEN

Publication

EP 1210451 A4 20040825 (EN)

Application

EP 00955193 A 20000817

Priority

  • NZ 0000159 W 20000817
  • NZ 33727699 A 19990817

Abstract (en)

[origin: WO0112844A1] The invention discloses a rapid method of measuring complex carbohydrates in mammalian tissue, said method comprising the steps of: extracting a sample of tissue to be tested; forming a homogenous slurry of the sample with an aqueous solution; adding sufficient hydrolysing enzyme for ensuring complete hydrolysis of glycogen in the slurry; and measuring the concentration of glucose in the slurry. The method can be conducted post-mortem to assay the concentration at the time of slaughter. The invention further discloses the measurement of lactate concentrations. The method of the invention can be conducted in thirty minutes or less.

IPC 1-7

C12Q 1/34; C12Q 1/54

IPC 8 full level

C12Q 1/34 (2006.01); C12Q 1/40 (2006.01)

CPC (source: EP)

C12Q 1/34 (2013.01); C12Q 1/40 (2013.01)

Citation (search report)

  • [Y] US 5262305 A 19931116 - HELLER ADAM [US], et al
  • [Y] EP 0086108 A2 19830817 - CHILDRENS HOSP MEDICAL CENTER [US]
  • [Y] BURTON SHELLEY A ET AL: "Evaluation of a glucose oxidase/peroxidase method for indirect measurement of glycogen content in marine mussels (Mytilus edulis)", JOURNAL OF SHELLFISH RESEARCH, vol. 16, no. 2, December 1997 (1997-12-01), pages 435 - 439, XP001182147, ISSN: 0730-8000
  • [Y] CARR R S ET AL: "Quantitative semi-automated enzymatic assay for tissue glycogen.", COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY. ENGLAND 1984, vol. 77, no. 3, 1984, pages 447 - 449, XP002286075, ISSN: 0305-0491
  • [Y] VOET AND VOET: "Biochemie", 1992, VCH, WEINHEIM, XP002286178
  • [T] BURTON SHELLEY A ET AL: "Evaluation of a glucose oxidase/peroxidase method for indirect measurement of glycogen content in oysters (Crassostrea virginica)", JOURNAL OF SHELLFISH RESEARCH, vol. 19, no. 2, December 2000 (2000-12-01), pages 841 - 844, XP001181700, ISSN: 0730-8000
  • See references of WO 0112844A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0112844 A1 20010222; AR 025303 A1 20021120; AU 6742500 A 20010313; AU 779415 B2 20050120; AU 779415 C 20060209; BR 0013395 A 20020709; CA 2382155 A1 20010222; EP 1210451 A1 20020605; EP 1210451 A4 20040825

DOCDB simple family (application)

NZ 0000159 W 20000817; AR P000104247 A 20000817; AU 6742500 A 20000817; BR 0013395 A 20000817; CA 2382155 A 20000817; EP 00955193 A 20000817