EP 1210596 A2 20020605 - METHODS FOR ASSAYING SUBCELLULAR CONDITIONS USING ENERGY TRANSFER
Title (en)
METHODS FOR ASSAYING SUBCELLULAR CONDITIONS USING ENERGY TRANSFER
Title (de)
VERFAHREN ZUR UNTERSUCHUNG SUBZELLULÄRER ZUSTÄNDE MIT HILFE VON ENERGIETRANSFER
Title (fr)
COMPOSITIONS ET PROCEDES D'ANALYSE DE CONDITIONS INFRACELLULAIRES ET TRAITEMENT PAR TRANSFERT D'ENERGIE
Publication
Application
Priority
- US 0017380 W 20000622
- US 33812299 A 19990622
- US 14043399 P 19990622
- US 17638300 P 20000114
Abstract (en)
[origin: WO0079274A2] The invention provides compositions and methods for monitoring subcellular compartments such as organelles by energy transfer techniques that do not require specific intermolecular affinity binding events between energy transfer donor and energy transfer acceptor molecules. Provided are methods for assaying cellular membrane potential, including mitochondrial membrane potential, by energy transfer methodologies including fluorescence resonance energy transfer (FRET). Diagnostic and drug screening assays are also provided.
IPC 1-7
G01N 33/50; G01N 33/58; G01N 33/542; C12N 15/72; C12Q 1/02; C12Q 1/04
IPC 8 full level
C12Q 1/00 (2006.01); G01N 21/64 (2006.01); G01N 33/15 (2006.01); G01N 33/48 (2006.01); G01N 33/483 (2006.01); G01N 33/50 (2006.01); G01N 33/542 (2006.01); G01N 33/58 (2006.01)
CPC (source: EP)
G01N 33/5005 (2013.01); G01N 33/5079 (2013.01); G01N 33/542 (2013.01); G01N 33/582 (2013.01); G01N 2500/00 (2013.01)
Citation (search report)
See references of WO 0079274A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0079274 A2 20001228; WO 0079274 A3 20020110; AU 5763600 A 20010109; CA 2375542 A1 20001228; EP 1210596 A2 20020605; JP 2003506014 A 20030218
DOCDB simple family (application)
US 0017380 W 20000622; AU 5763600 A 20000622; CA 2375542 A 20000622; EP 00943119 A 20000622; JP 2001505191 A 20000622