EP 1212406 A1 20020612 - CELL-FREE ASSAY FOR PLANT GENE TARGETING AND CONVERSION
Title (en)
CELL-FREE ASSAY FOR PLANT GENE TARGETING AND CONVERSION
Title (de)
ZELLFREIES TESTVERFAHREN FÜR ZIELGERICHTETE GENVERÄNDERUNGEN UND KONVERSIONEN VON PFLANZENGENEN
Title (fr)
ESSAI ACELLULAIRE POUR LE CIBLAGE ET LA CONVERSION DE GENE VEGETAL
Publication
Application
Priority
- US 0022974 W 20000821
- US 14998799 P 19990820
Abstract (en)
[origin: WO0114531A1] The invention is based on the reaction of oligonucleotides in a cell-free system containing a plant cell extract and a test duplex DNA on a plasmid. The reaction specifically corrects or causes a mutation in a selectable marker gene to a form that can be selected in transformed MutS and RecA deficient bacteria. After transformation into MutS and RecA deficient bacteria, the gene conversion can be detected and quantified.
IPC 1-7
IPC 8 full level
C12N 15/10 (2006.01); C12N 15/82 (2006.01)
CPC (source: EP US)
C12N 15/102 (2013.01 - EP US); C12N 15/8213 (2013.01 - EP US)
Citation (search report)
See references of WO 0114531A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0114531 A1 20010301; AU 6923300 A 20010319; EP 1212406 A1 20020612; US 2002193334 A1 20021219
DOCDB simple family (application)
US 0022974 W 20000821; AU 6923300 A 20000821; EP 00957644 A 20000821; US 8247602 A 20020220