EP 1221045 A2 20020710 - IMMUNO-CHROMATOGRAPHIC RAPID ASSAY IN ORDER TO DETECT ACID-RESISTANT MICROORGANISMS IN THE STOOL
Title (en)
IMMUNO-CHROMATOGRAPHIC RAPID ASSAY IN ORDER TO DETECT ACID-RESISTANT MICROORGANISMS IN THE STOOL
Title (de)
IMMUNCHROMATOGRAPHISCHER SCHNELLTEST ZUM NACHWEIS VON SÄURE-RESISTENTEN MIKROORGANISMEN IM STUHL
Title (fr)
TEST RAPIDE IMMUNO-CHROMATOGRAPHIQUE POUR LA DETECTION DE MICRO-ORGANISMES ACIDO-RESISTANTS DANS LES SELLES
Publication
Application
Priority
- EP 00972748 A 20001012
- EP 0010057 W 20001012
- EP 99120351 A 19991012
- EP 00105592 A 20000316
- EP 00107028 A 20000331
- EP 00110110 A 20000510
Abstract (en)
[origin: DE20023799U1] Detecting infection by an acid-resistant microorganism (A), in a mammal, comprises using immunochromatography. Detecting infection by an acid-resistant microorganism (A), in a mammal, comprises (a) preparing an immunochromatographic test strip having a sample application zone (I); (b) applying a fecal sample, containing an antigen (Ag) of (A) to (I); (c) incubating the sample with: (i) a first receptor (R1) to form a complex with Ag; or (ii) at least two different R1 to form a three-part complex with Ag, where R1 are specific for an Ag which, after passage through the intestines, at least in some mammals, retains a native (or corresponding) structure against which the mammal produces antibodies (when immunized or infected with (A), or its extracts, lysates or derived proteins (or fragments) or synthetic peptides); (d) immobilizing a second receptor (R2), able to bind to the complex formed between Ag and R1 to an analytical region; (e) transporting the first complex; (f) forming a second complex with R2 in the analytical region; and (g) detecting the complex. Independent claims are also included for the following: (1) an immunochromatography test device, especially for the new process, that comprises (I), an incubation system, an analytical region and a system for transporting the Ag-R1 complex; (2) monoclonal antibodies (MAb), their fragments or derivatives, that have a variable region comprising at least one of 24 specified CDRs (complementarity-determining regions), given in the specification; (3) an aptamer (i) that binds specifically to the same epitope as MAb; (4) an epitope (ii) that binds specifically to MAb or (i); (5) an antibody, or its fragments or derivatives, that bind specifically to (ii); and (6) a kit containing at least one test device of (1).
IPC 1-7
IPC 8 full level
C07K 16/40 (2006.01); G01N 33/48 (2006.01); G01N 33/569 (2006.01); G01N 33/573 (2006.01)
CPC (source: EP)
C07K 16/40 (2013.01); G01N 33/56911 (2013.01); G01N 33/573 (2013.01)
Citation (search report)
See references of WO 0127612A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
DOCDB simple family (application)
DE 20023799 U 20001012; EP 00972748 A 20001012