EP 1222202 A2 20020717 - AB5 TOXIN B SUBUNIT MUTANTS WITH ALTERED CHEMICAL CONJUG ATION CHARACTERISTICS
Title (en)
AB5 TOXIN B SUBUNIT MUTANTS WITH ALTERED CHEMICAL CONJUG ATION CHARACTERISTICS
Title (de)
MUTANTEN DER B UNTEREINHEIT DES AB5 TOXINS MIT VERÄNDERTER CHEMISCHER KONJUGATIONMERKMALE
Title (fr)
MUTANTS DE LA SOUS-UNITE B DE LA TOXINE AB 5? AUX CARACTERISTIQUES DE CONJUGAISON CHIMIQUE MODIFIEES
Publication
Application
Priority
- US 0027607 W 20001005
- US 15856199 P 19991008
Abstract (en)
[origin: WO0127144A2] A recombinant AB5 B subunit protein including at least one mutation, wherein the mutation alters the number of amino acid residues available for chemical modification as compared to a wild type AB5 B subunit protein, and wherein said recombinant protein retains an effective target ligand bind affinity. For example, specifically designed mutations are produced in the cholera Toxin B subunit (CTB) such that it can still bind with high affinity to its receptor, Gm-1, but can be specifically covalently linked at lysines or cysteines to an immunogen or vaccine. The vaccine produced from this coupling is a mucosal vaccine which has high immunogenicity due to the interaction with the CTB. The vaccine can be produced inexpensively and easily. Alternatively, a technique is disclosed for treating CTB such that non-covalent coupling to a vaccine or immunogen can occur.
IPC 1-7
C07K 14/28; C07K 14/245; C07K 14/25; C07K 14/235; C12N 15/31; C12N 5/10; A61K 39/106; A61K 39/112; A61K 39/10; A61K 39/108
IPC 8 full level
C12N 15/09 (2006.01); A61K 39/00 (2006.01); A61P 3/10 (2006.01); A61P 29/00 (2006.01); A61P 31/00 (2006.01); A61P 37/04 (2006.01); A61P 37/06 (2006.01); C07K 14/235 (2006.01); C07K 14/245 (2006.01); C07K 14/25 (2006.01); C07K 14/28 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP)
A61P 3/10 (2018.01); A61P 29/00 (2018.01); A61P 31/00 (2018.01); A61P 37/00 (2018.01); A61P 37/04 (2018.01); A61P 37/06 (2018.01); C07K 14/235 (2013.01); C07K 14/245 (2013.01); C07K 14/25 (2013.01); C07K 14/28 (2013.01); A61K 39/00 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0127144 A2 20010419; WO 0127144 A3 20020117; AU 7865900 A 20010423; CA 2385172 A1 20010419; EP 1222202 A2 20020717; IL 149009 A0 20021110; JP 2003511061 A 20030325; NZ 518342 A 20040430
DOCDB simple family (application)
US 0027607 W 20001005; AU 7865900 A 20001005; CA 2385172 A 20001005; EP 00968795 A 20001005; IL 14900900 A 20001005; JP 2001530362 A 20001005; NZ 51834200 A 20001005