EP 1222203 A1 20020717 - METHOD OF ALTERING POLYPEPTIDE AGGREGATION
Title (en)
METHOD OF ALTERING POLYPEPTIDE AGGREGATION
Title (de)
VERFAHREN ZUR VERÄNDERUNG DER POLYPEPTIDAGGREGATION
Title (fr)
ALTERATION DE L'AGREGATION DES POLYPEPTIDES
Publication
Application
Priority
- GB 0003974 W 20001016
- GB 9924484 A 19991015
Abstract (en)
[origin: WO0127152A1] The present invention provides a method of designing a modified polypeptide having an altered tendency to aggregate compared to the unmodified polypeptide. The method comprises: analysing the amino acid sequence of a predetermined polypeptide to determine the propensity of the polypeptide to form local structure; comparing the propensity to form local structure of a modified polypeptide to the propensity to form local structure of an unmodified polypeptide; and determining thereby whether the modified polypeptide has an altered tendency to aggregate in the denatured state relative to the unmodified polypeptide. A selected modified polypeptide having the altered tendency to aggregate is then produced. The invention also provides a method of producing a modified polypeptide having an altered tendency to aggregate compared to the unmodified polypeptide, which method comprises: (i) introducing at least one amino acid modification into a predetermined polypeptide sequence such that said modified polypeptide has an altered propensity to form local structure in the denatured state relative to the unmodified polypeptide, and optionally (ii) recovering the modified polypeptide, and/or optionally (iii) allowing the modified polypeptides to form an aggregate.
IPC 1-7
C07K 14/47; C07K 14/54; C12N 15/11; C12N 15/63; A61P 25/00; A61K 38/17
IPC 8 full level
C12N 15/02 (2006.01); A61K 31/711 (2006.01); A61K 35/00 (2006.01); A61K 35/76 (2006.01); A61K 38/00 (2006.01); A61K 48/00 (2006.01); A61P 3/10 (2006.01); A61P 11/00 (2006.01); A61P 25/00 (2006.01); A61P 25/14 (2006.01); A61P 25/16 (2006.01); A61P 25/28 (2006.01); A61P 37/00 (2006.01); A61P 43/00 (2006.01); C07K 14/54 (2006.01); C12N 9/12 (2006.01); C12N 9/14 (2006.01); C12N 9/48 (2006.01)
CPC (source: EP)
A61P 3/10 (2017.12); A61P 11/00 (2017.12); A61P 25/00 (2017.12); A61P 25/14 (2017.12); A61P 25/16 (2017.12); A61P 25/28 (2017.12); A61P 37/00 (2017.12); A61P 43/00 (2017.12); C07K 14/5406 (2013.01); C12N 9/1205 (2013.01); C12N 9/14 (2013.01); C12N 9/48 (2013.01); A61K 38/00 (2013.01)
Citation (search report)
See references of WO 0127152A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0127152 A1 20010419; AU 7807700 A 20010423; CA 2385123 A1 20010419; EP 1222203 A1 20020717; GB 9924484 D0 19991215; JP 2003512029 A 20030402
DOCDB simple family (application)
GB 0003974 W 20001016; AU 7807700 A 20001016; CA 2385123 A 20001016; EP 00968122 A 20001016; GB 9924484 A 19991015; JP 2001530370 A 20001016