EP 1222251 A4 20040707 - EX VIVO GENERATION OF FUNCTIONAL OSTEOCLASTS FROM BONE MARROW IN A THREE-DIMENSIONAL BIOREACTOR
Title (en)
EX VIVO GENERATION OF FUNCTIONAL OSTEOCLASTS FROM BONE MARROW IN A THREE-DIMENSIONAL BIOREACTOR
Title (de)
EX VIVO GEWINNUNG VON FUNKTIONELLEN OSTEOKLASTEN AUS DEM KNOCHENMARK IN EINEM DREIDIMENSIONALEN BIOREKTOR
Title (fr)
GENERATION EX VIVO DANS UN BIOREACTEUR TRIDIMENSIONNEL D'OSTEOCLASTES FONCTIONNELS DE MOELLE OSSEUSE
Publication
Application
Priority
- US 0022040 W 20000811
- US 14881499 P 19990813
Abstract (en)
[origin: WO0112785A1] The present invention provides cultured osteoclasts which function to resorb bone and methods for generating such osteoclasts. The method comprises isolating hemopoietic cells or accessory cells, culturing the hemopoietic cells or accessory cells in a chamber having a scaffolding covered or surrounded with culture medium, wherein the scaffolding allows for hemopoietic cells or accessory cells to have cell to cell contacts in three dimensions. The subject osteoclasts are useful for screening drugs which inhibit or stimulate osteoclastogenesis and/or osteoclast function. The subject osteoclasts are also useful in treating certain bone diseases and for use in bone remodeling and regeneration.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); A61K 35/28 (2015.01); A61P 3/14 (2006.01); A61P 7/00 (2006.01); A61P 19/00 (2006.01); A61P 19/10 (2006.01); A61P 43/00 (2006.01); C12N 5/078 (2010.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); G01N 33/15 (2006.01); G01N 33/53 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP)
A61P 3/14 (2018.01); A61P 7/00 (2018.01); A61P 19/00 (2018.01); A61P 19/10 (2018.01); A61P 43/00 (2018.01); C12N 5/0643 (2013.01); A61K 35/12 (2013.01); C12N 2500/32 (2013.01); C12N 2500/38 (2013.01); C12N 2501/37 (2013.01); C12N 2501/39 (2013.01); C12N 2503/02 (2013.01); C12N 2531/00 (2013.01); C12N 2533/76 (2013.01); C12N 2533/78 (2013.01); G01N 2500/10 (2013.01)
Citation (search report)
- [X] LACEY D L ET AL: "OSTEOPROTEGERIN LIGAND IS A CYTOKINE THAT REGULATES OSTEOCLAST DIFFERENTIATION AND ACTIVATION", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 93, 17 April 1998 (1998-04-17), pages 165 - 176,1, XP002934582, ISSN: 0092-8674
- [X] UDAGAWA N ET AL: "ORIGIN OF OSTEOCLASTS MATURE MONOCYTES AND MACROPHAGES ARE CAPABLE OF DIFFERENTIATING INTO OSTEOCLASTS UNDER A SUITABLE MICROENVIRONMENT PREPARED BY BONE MARROW-DERIVED STROMAL CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 87, no. 18, 1990, pages 7260 - 7264, XP002199542, ISSN: 0027-8424
- [X] DE BRUIJN J D ET AL: "Osteoclastic resorption of calcium phosphates is potentiated in postosteogenic culture conditions", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, vol. 28, no. 1, 1994, pages 105 - 112, XP009024411, ISSN: 0021-9304
- [X] SATO T ET AL: "THE MIGRATION OF PURIFIED OSTEOCLASTS THROUGH COLLAGEN IS INHIBITEDBY MATRIX METALLOPROTEINASE INHIBITORS", JOURNAL OF BONE AND MINERAL RESEARCH, NEW YORK, NY, US, vol. 13, no. 1, January 1998 (1998-01-01), pages 59 - 66, XP000992863, ISSN: 0884-0431
- [Y] WANG T-Y BRENNAN J K ET AL: "MULTILINEAL HEMATOPOIESIS IN A THREE-DIMENSIONAL MURINE LONG-TERM BONE MARROW CULTURE", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 23, 1995, pages 26 - 32, XP002944956, ISSN: 0301-472X
- [Y] MANTALARIS A ET AL: "ENGINEERING A HUMAN BONE MARROW MODEL: A CASE STUDY ON EX VIVO ERYTHROPOIESIS", BIOTECHNOLOGY PROGRESS, XX, XX, vol. 14, no. 1, 1998, pages 126 - 133, XP002944957, ISSN: 8756-7938
- See also references of WO 0112785A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0112785 A1 20010222; AU 6766100 A 20010313; EP 1222251 A1 20020717; EP 1222251 A4 20040707; JP 2003507035 A 20030225
DOCDB simple family (application)
US 0022040 W 20000811; AU 6766100 A 20000811; EP 00955457 A 20000811; JP 2001517670 A 20000811