EP 1222261 A2 20020717 - ADJUSTABLE SENSITIVITY, GENETIC MOLECULAR INTERACTION SYSTEMS, INCLUDING PROTEIN-PROTEIN INTERACTION SYSTEMS FOR DETECTION AND ANALYSIS
Title (en)
ADJUSTABLE SENSITIVITY, GENETIC MOLECULAR INTERACTION SYSTEMS, INCLUDING PROTEIN-PROTEIN INTERACTION SYSTEMS FOR DETECTION AND ANALYSIS
Title (de)
TESTSYSTEME FÜR MOLEKULARE WECHSELWIRKUNGEN MIT EINSTELLBARER EMPFINDLICHKEIT, EINSCHLIESSLICH TESTSYSTEMEN FÜR PROTEIN-PROTEIN WECHSELWIRKUNGEN, ZU NACHWEIS- UND ANALYSEZWECKEN
Title (fr)
SYSTEMES D'INTERACTIONS GENETIQUES MOLECULAIRES A SENSIBILITE REGLABLE COMPORTANT DES SYSTEMES D'INTERACTIONS PROTEINE-PROTEINE DE DETECTION ET D'ANALYSE
Publication
Application
Priority
- US 0027677 W 20001006
- US 15807999 P 19991007
Abstract (en)
[origin: WO0125420A2] A method for detecting interactions between first and second interacting molecules at variable sensitivity. This variable sensitivity may be obtained by providing for the overexpression of either a bait hybrid protein containing a DNA binding domain (desensitization) or a prey hybrid protein containing the DNA activation domain for a reporter gene (enhanced sensitivity). The use of exogenous activators of one or the other according to the needs of a particular system is readily accomplished.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6897 (2018.01)
CPC (source: EP)
C12N 15/1055 (2013.01); C12Q 1/6897 (2013.01)
Citation (search report)
See references of WO 0125420A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0125420 A2 20010412; WO 0125420 A3 20020418; AU 1074401 A 20010510; CA 2386258 A1 20010412; EP 1222261 A2 20020717; IL 148967 A0 20021110; JP 2004500805 A 20040115
DOCDB simple family (application)
US 0027677 W 20001006; AU 1074401 A 20001006; CA 2386258 A 20001006; EP 00972024 A 20001006; IL 14896700 A 20001006; JP 2001528573 A 20001006