EP 1224260 A2 20020724 - METHODS FOR IMPROVING VIRAL VECTOR TITERS AND REDUCING CELL DEATH IN CELL CULTURES
Title (en)
METHODS FOR IMPROVING VIRAL VECTOR TITERS AND REDUCING CELL DEATH IN CELL CULTURES
Title (de)
VERFAHREN ZUR ERHÖHUNG VON VIRUSVEKTORTITERN UND ZUR REDUKTION DES ZELLTODES IN ZELLKULTUREN
Title (fr)
PROCEDES PERMETTANT D'AMELIORER LES TITRES DE VECTEUR VIRAUX ET DE REDUIRE LA MORT CELLULAIRE DANS DES CULTURES CELLULAIRES
Publication
Application
Priority
- US 0029549 W 20001027
- US 16234699 P 19991029
Abstract (en)
[origin: WO0132838A2] Disclosed are compositions and methods for the increasing the production of a recombinant viral vector in a cell culture, comprising culturing a recombinant viral vector producing cell line in a basal media which further comprises (a) greater than 1.5 g/L amino acids (or, a selected amino acids in a quantity and amount greater than present in a basal media such as DMEM), and (b) between 0.5% and 4% serum, such that the recombinant viral vectors are produced.
IPC 1-7
IPC 8 full level
C12N 5/02 (2006.01); C12N 5/00 (2006.01); C12N 15/86 (2006.01); C12N 15/861 (2006.01); C12N 15/864 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C12N 5/0018 (2013.01); C12N 15/86 (2013.01); C12N 2500/20 (2013.01); C12N 2500/32 (2013.01); C12N 2710/10343 (2013.01); C12N 2740/13043 (2013.01); C12N 2750/14143 (2013.01); C12N 2770/36143 (2013.01); C12N 2800/30 (2013.01)
Citation (search report)
See references of WO 0132838A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0132838 A2 20010510; WO 0132838 A3 20020117; AU 1346501 A 20010514; CA 2389057 A1 20010510; EP 1224260 A2 20020724; JP 2003513623 A 20030415
DOCDB simple family (application)
US 0029549 W 20001027; AU 1346501 A 20001027; CA 2389057 A 20001027; EP 00975409 A 20001027; JP 2001535520 A 20001027