EP 1230258 A1 20020814 - MULTI-COMPARTMENT ELECTROPHORESIS
Title (en)
MULTI-COMPARTMENT ELECTROPHORESIS
Title (de)
MULTI-KOMPARTIMENT-ELEKTROPHORESE
Title (fr)
APPAREIL D'ELECTROPHORESE A PLUSIEURS COMPARTIMENTS
Publication
Application
Priority
- AU 0001391 W 20001114
- AU PQ405899 A 19991115
- AU PQ872700 A 20000712
Abstract (en)
[origin: WO0136449A1] A novel multi-compartment electrolyser design is used to prefractionate complex protein mixtures, for use prior to the implementation of 2-D maps. Such sub-fractionation processes can effectively remove, via suitable narrow range isoelectric membranes, proteins present in large excess in a cell lysate or in body fluids. In turn, the remaining protein mixture, devoid of such major components, can be loaded in a 2-D map at much higher levels, thus ensuring a greater sensitivity and detection capability of low-abundance proteins. The apparatus is a multi-compartment electrolyser, which is characterised in that recirculation of the samples is not required. The apparatus produces protein fractions which are fully compatible with the subsequent 2-D protocols, since it is based on a focusing technique, which yields samples highly concentrated and devoid of salts and buffers.
IPC 1-7
IPC 8 full level
G01N 33/483 (2006.01); B01D 57/02 (2006.01); C07K 1/26 (2006.01); C07K 1/28 (2006.01); G01N 1/10 (2006.01); G01N 27/447 (2006.01)
CPC (source: EP)
B01D 57/02 (2013.01); C07K 1/26 (2013.01); G01N 27/44708 (2013.01); G01N 27/44795 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0136449 A1 20010525; EP 1230258 A1 20020814; EP 1230258 A4 20041222; HK 1049343 A1 20030509; JP 2003514829 A 20030422
DOCDB simple family (application)
AU 0001391 W 20001114; EP 00974180 A 20001114; HK 03100703 A 20030128; JP 2001538938 A 20001114