EP 1231993 A4 20030416 - IMPROVED COLUMN FOR DNA SEPARATION BY MATCHED ION POLYNUCLEOTIDE CHROMATOGRAPHY
Title (en)
IMPROVED COLUMN FOR DNA SEPARATION BY MATCHED ION POLYNUCLEOTIDE CHROMATOGRAPHY
Title (de)
VERBESSERTE KOLONNE ZUR DNA-TRENNUNG DURCH POLYNUKLEOTIDCHROMATOGRPHIE MIT AUFEINANDER ABGESTIMMTEN IONEN
Title (fr)
COLONNE AMELIOREE POUR LA SEPARATION DE L'ADN EN CHROMATOGRAPHIE DE NUCLEOTIDES A IONS APPARIES (MIPC)
Publication
Application
Priority
- US 0025532 W 20000918
- US 15461499 P 19990917
- US 15874399 P 19991012
- US 16751599 P 19991124
- US 17717700 P 20000120
- US 50177500 A 20000210
Abstract (en)
[origin: WO0119485A1] An improved separation column and method for separating a mixture of double stranded DNA fragments by Matched Ion Polynucleotide Chromatography. The cylindrical column has an ID greater than about 5 mm and contains polymer beads. The beads have an average diameter of 1 to 100 microns and are unsubstituted polymer beads or are polymer beads substituted with a hydrocarbon moiety having from 1 to 1,000,000 carbons. The preferred beads are characterized by being substantially free from multivalent cations which are free to bind with DNA. The improved column provides enhanced separation of DNA fragments with sizes ranging from about 100 to 20,000 base pairs. The column also provides enhanced separation of heteroduplex and homoduplex DNA molecules in a mutation detection procedure in which the chromatography is performed under conditions effecting partial denaturation of DNA at a site of mismatched base pairs.
IPC 1-7
IPC 8 full level
B01J 20/281 (2006.01); B01D 15/08 (2006.01); B01D 15/36 (2006.01); B01J 20/285 (2006.01); C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 30/34 (2006.01); G01N 30/60 (2006.01); G01N 30/88 (2006.01); B01D 15/16 (2006.01); B01D 15/20 (2006.01); B01D 15/22 (2006.01); G01N 30/02 (2006.01); G01N 30/30 (2006.01)
CPC (source: EP US)
B01D 15/366 (2013.01 - EP US); B01J 20/265 (2013.01 - EP); B01J 20/285 (2013.01 - EP US); B01D 15/166 (2013.01 - EP); B01D 15/20 (2013.01 - EP); B01D 15/22 (2013.01 - EP); G01N 2030/3084 (2013.01 - EP)
C-Set (source: EP)
Citation (search report)
- [X] WO 9907899 A1 19990218 - TRANSGENOMIC INC [US], et al
- [X] WO 9856797 A1 19981217 - TRANSGENOMIC INC [US], et al
- See references of WO 0119485A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT
DOCDB simple family (publication)
WO 0119485 A1 20010322; AU 7384100 A 20010417; CA 2375746 A1 20010322; EP 1231993 A1 20020821; EP 1231993 A4 20030416; JP 2003509662 A 20030311
DOCDB simple family (application)
US 0025532 W 20000918; AU 7384100 A 20000918; CA 2375746 A 20000918; EP 00961961 A 20000918; JP 2001523107 A 20000918