EP 1235634 A1 20020904 - SOLUTION BASED TWO-DIMENSIONAL SEPARATION AND DETECTION OF AMPHOTERIC SUBSTANCES
Title (en)
SOLUTION BASED TWO-DIMENSIONAL SEPARATION AND DETECTION OF AMPHOTERIC SUBSTANCES
Title (de)
ZWEIDIMENSIONALE TRENNUNG UND DETEKTION VON IN LÖSUNG BEFINDLICHEN AMPHOTEREN SUBSTANZEN
Title (fr)
SEPARATION ET DETECTION BIDIMENSIONNELLES EN SOLUTION DE SUBSTANCES AMPHOTERES
Publication
Application
Priority
- US 0031050 W 20001114
- US 16565999 P 19991116
Abstract (en)
[origin: WO0136071A1] A method and apparatus for separating mixtures of amphoteric substances uses two dimensions of separation. A sample valve (8, 9) is used to introduce a sample into the mobile phase (7b) for entry into the first dimension of separation, typically a chromatography column (10). The separated sample is passed to the second dimension, which is formed by a series of stages (11, 18, 19). The first stage (11) has a manifold having an anode (13), cathode (12) and a membrane (15) of a specific pK, defining two channels (16a, 16b). The amphoteric molecules are segregated between the two channels and the segregated molecules are passed to a second stage, where each channel (16a, 16b) is further split into two channels (16e, 16f and 16c, 16d), respectively. In each subsequent stage (19), the number of channels is doubled from the previous stage, increasing the degree of separation by pH.
IPC 1-7
IPC 8 full level
B01D 57/02 (2006.01); G01N 27/447 (2006.01); G01N 30/46 (2006.01); G01N 30/02 (2006.01)
CPC (source: EP)
B01D 57/02 (2013.01); G01N 27/44743 (2013.01); G01N 27/44769 (2013.01); G01N 30/461 (2013.01); G01N 30/02 (2013.01); G01N 30/463 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE FR GB IT LI
DOCDB simple family (publication)
WO 0136071 A1 20010525; AU 1599501 A 20010530; EP 1235634 A1 20020904; EP 1235634 A4 20040901
DOCDB simple family (application)
US 0031050 W 20001114; AU 1599501 A 20001114; EP 00978541 A 20001114