EP 1235920 A2 20020904 - DOWN-REGULATION AND SILENCING OF ALLERGEN GENES IN TRANSGENIC PEANUT SEEDS
Title (en)
DOWN-REGULATION AND SILENCING OF ALLERGEN GENES IN TRANSGENIC PEANUT SEEDS
Title (de)
HERABREGULIERUNG UND ABSCHALTUNG VON ALLERGENEN GENEN IN TRANSGENEN ERDNUSSSAMEN
Title (fr)
REGULATION NEGATIVE ET INHIBITION DES GENES ALLERGENES DANS DES GRAINES D'ARACHIDE TRANSGENIQUES
Publication
Application
Priority
- US 0031657 W 20001120
- US 16725599 P 19991119
Abstract (en)
[origin: WO0136621A2] An allergen-free transgenic peanut seed is produced by recombinant methods. Peanut plants are transformed with multiple copies of each of the allergen genes, or fragments thereof, to suppress gene expression and allergen protein production. Alternatively, peanut plants are transformed with peanut allergen antisense genes introduced into the peanut genome as antisense fragments, sense fragments, or combinations of both antisense and sense fragments. Peanut transgenes are under the control of the 35S promoter, or the promoter of the Ara h2 gene to produce antisense RNAs, sense RNAs, and double-stranded RNAs for suppressing allergen protein production in peanut plants. A full length genomic clone for allergen Ara h2 is isolated and sequenced. The ORF is 622 nucleotides long. The predicted encoded protein is 207 amino acids long and includes a putative transit peptide of 21 residues. One polyadenilation signal is identified at position 951. Six additional stop codons are observed. A promoter region was revealed containing a putative TATA box located at position -72. Homologous regions were identified between Ara h2, h6, and h7, and between Ara h3 and h4, and between Ara h1P41B and Ara h1P17. The homologous regions will be used for the screening of peanut genomic library to isolate all peanut allergen genes and for down-regulation and silencing of multiple peanut allergen genes.
IPC 1-7
IPC 8 full level
C07K 14/415 (2006.01); C12N 15/82 (2006.01)
CPC (source: EP)
C07K 14/415 (2013.01); C12N 15/8242 (2013.01); C12N 15/8251 (2013.01)
Citation (search report)
See references of WO 0136621A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0136621 A2 20010525; WO 0136621 A3 20020110; AU 1921601 A 20010530; EP 1235920 A2 20020904
DOCDB simple family (application)
US 0031657 W 20001120; AU 1921601 A 20001120; EP 00982149 A 20001120