Global Patent Index - EP 1235920 A2

EP 1235920 A2 2002-09-04 - DOWN-REGULATION AND SILENCING OF ALLERGEN GENES IN TRANSGENIC PEANUT SEEDS

Title (en)

DOWN-REGULATION AND SILENCING OF ALLERGEN GENES IN TRANSGENIC PEANUT SEEDS

Title (de)

HERABREGULIERUNG UND ABSCHALTUNG VON ALLERGENEN GENEN IN TRANSGENEN ERDNUSSSAMEN

Title (fr)

REGULATION NEGATIVE ET INHIBITION DES GENES ALLERGENES DANS DES GRAINES D'ARACHIDE TRANSGENIQUES

Publication

EP 1235920 A2 (EN)

Application

EP 00982149 A

Priority

  • US 0031657 W
  • US 16725599 P

Abstract (en)

[origin: WO0136621A2] An allergen-free transgenic peanut seed is produced by recombinant methods. Peanut plants are transformed with multiple copies of each of the allergen genes, or fragments thereof, to suppress gene expression and allergen protein production. Alternatively, peanut plants are transformed with peanut allergen antisense genes introduced into the peanut genome as antisense fragments, sense fragments, or combinations of both antisense and sense fragments. Peanut transgenes are under the control of the 35S promoter, or the promoter of the Ara h2 gene to produce antisense RNAs, sense RNAs, and double-stranded RNAs for suppressing allergen protein production in peanut plants. A full length genomic clone for allergen Ara h2 is isolated and sequenced. The ORF is 622 nucleotides long. The predicted encoded protein is 207 amino acids long and includes a putative transit peptide of 21 residues. One polyadenilation signal is identified at position 951. Six additional stop codons are observed. A promoter region was revealed containing a putative TATA box located at position -72. Homologous regions were identified between Ara h2, h6, and h7, and between Ara h3 and h4, and between Ara h1P41B and Ara h1P17. The homologous regions will be used for the screening of peanut genomic library to isolate all peanut allergen genes and for down-regulation and silencing of multiple peanut allergen genes.

IPC 1-7 (main, further and additional classification)

C12N 15/82; C07K 14/415; C12N 15/29

IPC 8 full level (invention and additional information)

C07K 14/415 (2006.01); C12N 15/82 (2006.01)

CPC (invention and additional information)

C07K 14/415 (2013.01); C12N 15/8242 (2013.01); C12N 15/8251 (2013.01)

Citation (search report)

See references of WO 0136621A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0136621 A2 20010525; WO 0136621 A3 20020110; AU 1921601 A 20010530; EP 1235920 A2 20020904

INPADOC legal status


2004-05-19 [18W] APPLICATION WITHDRAWN

- Effective date: 20040329

2002-09-04 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020618

2002-09-04 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2002-09-04 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI