Global Patent Index - EP 1238091 A2

EP 1238091 A2 2002-09-11 - METHODS AND COMPOSITIONS FOR THE MANUFACTURE OF REPLICATION INCOMPETENT ADENOVIRUS

Title (en)

METHODS AND COMPOSITIONS FOR THE MANUFACTURE OF REPLICATION INCOMPETENT ADENOVIRUS

Title (de)

VERFAHREN UND ZUSAMMENSETZUNGEN ZUR HERSTELLUNG VON REPLICATIONSUNFÄHIGEN ADENOVIRUS

Title (fr)

METHODES ET COMPOSITIONS DESTINEES A LA FABRICATION D'ADENOVIRUS INCOMPETENTS POUR LA REPLICATION

Publication

EP 1238091 A2 (EN)

Application

EP 00980987 A

Priority

  • US 0033123 W
  • US 17055099 P
  • US 19626600 P

Abstract (en)

[origin: WO0144280A2] The present invention relates to complementing cell lines for the production of replication incompetent viruses, which significantly reduce or eliminate the presence of replication competent viruses. Methods to make and use the complementing cell lines are also provided, as are nucleic acid molecules, polynucleotides, and vectors for making the cell lines. In particular, the present invention relates to complementing cell lines for the production of replication incompetent adenoviruses (Ad), which significantly reduce or eliminate the presence of replication competent Ad (RCA) and can serve for the large scale production of infectious replication incompetent adenovirus particles that may be used for the treatment of human patients as for example in gene therapy. The present invention further relates to an assay for detecting the presence of replication competent virus particles, in particular RCA, in a stock of infectious replication incompetent virus particles, in particular replication incompetent adenovirus particles, which employs a real time quantitative PCR assay with a sensitivity level to detect one replication competent virus particle per ≥ 10<9> replication incompetent virus particles.

IPC 1-7 (main, further and additional classification)

C12N 15/86

IPC 8 full level (invention and additional information)

G01N 33/53 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/861 (2006.01); C12Q 1/68 (2006.01); G01N 33/566 (2006.01); G01N 33/569 (2006.01)

CPC (invention and additional information)

C12N 15/86 (2013.01); C12N 2710/10343 (2013.01); C12N 2710/10351 (2013.01); C12N 2840/203 (2013.01); C12N 2840/44 (2013.01)

Citation (search report)

See references of WO 0144280A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0144280 A2 20010621; WO 0144280 A3 20011129; AU 1817401 A 20010625; CA 2393844 A1 20010621; EP 1238091 A2 20020911; JP 2003516743 A 20030520

INPADOC legal status


2004-08-25 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20040212

2003-11-19 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031001

2002-12-11 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: HIMES, VAUGHN, B.

2002-12-11 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: RASTY, SIYAMAK

2002-12-11 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: PELUSO, RICHARD, W.

2002-09-11 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020620

2002-09-11 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2002-09-11 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI