EP 1238091 A2 20020911 - METHODS AND COMPOSITIONS FOR THE MANUFACTURE OF REPLICATION INCOMPETENT ADENOVIRUS
Title (en)
METHODS AND COMPOSITIONS FOR THE MANUFACTURE OF REPLICATION INCOMPETENT ADENOVIRUS
Title (de)
VERFAHREN UND ZUSAMMENSETZUNGEN ZUR HERSTELLUNG VON REPLICATIONSUNFÄHIGEN ADENOVIRUS
Title (fr)
METHODES ET COMPOSITIONS DESTINEES A LA FABRICATION D'ADENOVIRUS INCOMPETENTS POUR LA REPLICATION
Publication
Application
Priority
- US 0033123 W 20001207
- US 17055099 P 19991214
- US 19626600 P 20000411
Abstract (en)
[origin: WO0144280A2] The present invention relates to complementing cell lines for the production of replication incompetent viruses, which significantly reduce or eliminate the presence of replication competent viruses. Methods to make and use the complementing cell lines are also provided, as are nucleic acid molecules, polynucleotides, and vectors for making the cell lines. In particular, the present invention relates to complementing cell lines for the production of replication incompetent adenoviruses (Ad), which significantly reduce or eliminate the presence of replication competent Ad (RCA) and can serve for the large scale production of infectious replication incompetent adenovirus particles that may be used for the treatment of human patients as for example in gene therapy. The present invention further relates to an assay for detecting the presence of replication competent virus particles, in particular RCA, in a stock of infectious replication incompetent virus particles, in particular replication incompetent adenovirus particles, which employs a real time quantitative PCR assay with a sensitivity level to detect one replication competent virus particle per ≥ 10<9> replication incompetent virus particles.
IPC 1-7
IPC 8 full level
C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/861 (2006.01); C12Q 1/68 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/569 (2006.01)
CPC (source: EP)
C12N 15/86 (2013.01); C12N 2710/10343 (2013.01); C12N 2710/10351 (2013.01); C12N 2840/203 (2013.01); C12N 2840/44 (2013.01)
Citation (search report)
See references of WO 0144280A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0144280 A2 20010621; WO 0144280 A3 20011129; AU 1817401 A 20010625; CA 2393844 A1 20010621; EP 1238091 A2 20020911; JP 2003516743 A 20030520
DOCDB simple family (application)
US 0033123 W 20001207; AU 1817401 A 20001207; CA 2393844 A 20001207; EP 00980987 A 20001207; JP 2001544769 A 20001207