EP 1238109 A2 20020911 - METHODS AND KITS FOR DISCOVERY OF RNA-BINDING ANTIMICROBIALS
Title (en)
METHODS AND KITS FOR DISCOVERY OF RNA-BINDING ANTIMICROBIALS
Title (de)
METHODEN UND TESTSÄTZE ZUR ENTDECKUNG VON RNA-BINDENDEN ANTIMIKROBIELLEN SUBSTANZEN
Title (fr)
PROCEDES ET KITS PERMETTANT DE DECOUVRIR DES ANTIMICROBIENS SE LIANT A L'ARN
Publication
Application
Priority
- GB 0004844 W 20001215
- GB 9929810 A 19991216
- US 46535599 A 19991216
Abstract (en)
[origin: WO0144505A2] The invention provides a method for determining whether a test compound binds to a target RNA, the method comprising the steps of: a) contacting the test compound with a pair of indicator molecules comprising an antimicrobial labelled with a donor group or an acceptor group and the target RNA labelled with a complementary acceptor or donor group, the pair being capable of binding to each other in an orientation that permits the donor group to come into sufficient proximity to the acceptor group to permit fluorescent resonance energy transfer and/or quenching to take place; and b) measuring the fluorescence of the target RNA and/or the antimicrobial in the presence of the test compound and comparing this value to the fluorescence of a standard.
IPC 1-7
IPC 8 full level
C07H 21/02 (2006.01); C07K 7/08 (2006.01); C07K 7/64 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6818 (2013.01); C12Q 1/689 (2013.01)
Citation (search report)
See references of WO 0144505A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0144505 A2 20010621; WO 0144505 A3 20011220; AU 2200401 A 20010625; EP 1238109 A2 20020911; GB 0030862 D0 20010131; GB 2359357 A 20010822; GB 2359357 A8 20020304
DOCDB simple family (application)
GB 0004844 W 20001215; AU 2200401 A 20001215; EP 00985595 A 20001215; GB 0030862 A 20001218