EP 1238112 A2 20020911 - METHOD FOR THE PARALLEL DETECTION OF THE DEGREE OF METHYLATION OF GENOMIC DNA
Title (en)
METHOD FOR THE PARALLEL DETECTION OF THE DEGREE OF METHYLATION OF GENOMIC DNA
Title (de)
VERFAHREN ZUR PARALLELEN DETEKTION DES METHYLIERUNGSZUSTANDES VON GENOMISCHER DNA
Title (fr)
PROCEDE DE DETECTION PARALLELE DE L'ETAT DE METHYLATION D'ADN GENOMIQUE
Publication
Application
Priority
- DE 0004381 W 20001206
- DE 19959691 A 19991206
Abstract (en)
[origin: WO0142493A2] The invention relates to a method for the parallel detection of the degree of methylation of genomic DNA wherein the following the steps are performed: (a) chemical treatment at the 5' position of non-methylated cytosine bases converts said bases into uracil, thymidine or another base which exhibits hybridization behavior different to that of cytosine in a genomic DNA sample; (b) more than ten different fragments, each having less than 2000 base pairs in said chemically treated genomic DNA sample, are amplified simultaneously using synthetic oligonucleotides as a primer, whereby said primers each contain genomic sequences which are involved in gene regulation and/or transcribed and/or translated, such as those sequences which should be obtained after execution of steps (a); (c) the sequence contexts of all or a portion of the CpG dinucleotides or CpNpG trinucleotides contained in the amplified fragments are determined.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6858 (2018.01)
CPC (source: EP US)
C12Q 1/6858 (2013.01 - EP US); C12Q 2600/156 (2013.01 - EP US)
Citation (search report)
See references of WO 0142493A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0142493 A2 20010614; WO 0142493 A3 20020207; AU 2663201 A 20010618; AU 778411 B2 20041202; CA 2395047 A1 20010614; DE 10083729 D2 20030515; DE 19959691 A1 20010816; EP 1238112 A2 20020911; US 2004248090 A1 20041209
DOCDB simple family (application)
DE 0004381 W 20001206; AU 2663201 A 20001206; CA 2395047 A 20001206; DE 10083729 T 20001206; DE 19959691 A 19991206; EP 00989842 A 20001206; US 14910902 A 20021024