EP 1239288 A1 20020911 - Identification of nucleotides by mass spectrometry
Title (en)
Identification of nucleotides by mass spectrometry
Title (de)
Identifizierung von Nukleotiden durch Massenspektrometrie
Title (fr)
Identification de nucléotides par spectrometrie de masse
Publication
Application
Priority
- EP 95914051 A 19950314
- US 21243394 A 19940314
Abstract (en)
A method for correlating a peptide fragment mass spectrum with amino acid sequences derived from a database is provided. A peptide is analyzed by a tandem mass spectrometer to yield a peptide fragment mass spectrum. A protein sequence database or a nucleotide sequence database is used to predict one or more fragment spectra for comparison with the experimentally-derived fragment spectrum. In one embodiment, sub-sequences of the sequences found on the database which define a peptide having a mass substantially equal to the mass of the peptide analyzed by the tandem mass spectrometer are identified as candidate sequences. For each candidate sequence, a plurality of fragments of the sequence are identified and the masses and m/z ratios of the fragments are predicted and used to form a predicted mass spectrum. The various predicted mass spectra are compared to the experimentally derived fragment spectrum using a closeness-of-fit measure, preferably calculated with a two-step process, including a calculation of a preliminary score end, for the highest-scoring predicted spectra, calculation of a correlation function.
IPC 1-7
IPC 8 full level
G01N 27/62 (2006.01); C07K 1/00 (2006.01); C07K 1/04 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6816 (2018.01); G01N 30/72 (2006.01); G01N 33/50 (2006.01); G01N 33/66 (2006.01); G01N 33/68 (2006.01); G16B 40/10 (2019.01); H01J 49/02 (2006.01); H01J 49/04 (2006.01); H01J 49/34 (2006.01)
CPC (source: EP US)
C07K 1/00 (2013.01 - EP US); C07K 1/047 (2013.01 - EP US); C12Q 1/6816 (2013.01 - EP US); G01N 33/66 (2013.01 - EP US); G01N 33/6818 (2013.01 - EP US); G01N 33/6848 (2013.01 - EP US); G16B 40/10 (2019.01 - EP US); G16B 99/00 (2019.01 - EP US); G16C 20/20 (2019.01 - EP US); H01J 49/0036 (2013.01 - EP US); H01J 49/004 (2013.01 - EP US); G01N 30/7233 (2013.01 - EP US); Y10T 436/143333 (2015.01 - EP US); Y10T 436/24 (2015.01 - EP US); Y10T 436/25125 (2015.01 - EP US)
Citation (search report)
- [A] DATABASE WPI Section EI Week 8438, Derwent World Patents Index; Class S03, AN 84-233469, XP002029487
- [A] DATABASE WPI Section Ch Week 8822, Derwent World Patents Index; Class B04, AN 88-151045, XP002029488
- [PX] J.K. ENG ET AL.: "An approach to correlate tandem mass-spectral data of peptides with amino-acid-sequences in a protein database", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 5, no. 11, November 1994 (1994-11-01), NEW YORK US, pages 976 - 989, XP000197296
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
EP 1239288 A1 20020911; AT E241809 T1 20030615; CA 2185574 A1 19950921; CA 2185574 C 20030603; DE 69530915 D1 20030703; DE 69530915 T2 20040311; DK 0750747 T3 20030929; EP 0750747 A1 19970102; EP 0750747 A4 19970625; EP 0750747 B1 20030528; ES 2199986 T3 20040301; JP 3195358 B2 20010806; JP H09510780 A 19971028; US 5538897 A 19960723; US 6017693 A 20000125; WO 9525281 A1 19950921
DOCDB simple family (application)
EP 02012695 A 19950314; AT 95914051 T 19950314; CA 2185574 A 19950314; DE 69530915 T 19950314; DK 95914051 T 19950314; EP 95914051 A 19950314; ES 95914051 T 19950314; JP 52415795 A 19950314; US 21243394 A 19940314; US 71625696 A 19961127; US 9503239 W 19950314