EP 1240316 A2 20020918 - RESOLVASE-CATALYSED, SEQUENCE-SPECIFIC DNA-RECOMBINATION IN EUKARYOTIC CELLS
Title (en)
RESOLVASE-CATALYSED, SEQUENCE-SPECIFIC DNA-RECOMBINATION IN EUKARYOTIC CELLS
Title (de)
RESOLVASE-KATALYSIERTE, SEQUENZ-SPEZIFISCHE DNA-REKOMBINATION IN EUKARYOTISCHEN ZELLEN
Title (fr)
RECOMBINAISON AVEC UNE SEQUENCE SPECIFIQUE D'ADN CATALYSEE PAR RESOLVASE DANS DES CELLULES EUCARYOTES
Publication
Application
Priority
- DE 19956513 A 19991124
- EP 0011718 W 20001124
Abstract (en)
[origin: WO0138511A2] The invention relates to a method for the sequence-specific recombination of DNA in eukaryotic cells, comprising the following steps: introducing a first DNA sequence which contains a res sequence into a cell, introducing a copy of the first DNA sequence and performing the sequence-specific recombination using the influence of a resolvase or its derivative. The invention also relates to a method for testing topoisomerase inhibitors in a eukaryotic cell, comprising the following steps: introducing a first DNA sequence according to SEQ ID NO:1 into a cell, introducing a copy of the first DNA sequence according to SEQ ID NO:1 in direct orientation, introducing a wild-type resolvase and adding the topoisomerase inhibitors which are to be tested.
IPC 1-7
IPC 8 full level
A01H 5/00 (2006.01); A01K 67/027 (2006.01); A01K 67/033 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 5/10 (2006.01); C12N 9/00 (2006.01); C12N 9/10 (2006.01); C12N 9/22 (2006.01); C12N 15/09 (2006.01); C12N 15/90 (2006.01); C12Q 1/02 (2006.01)
CPC (source: EP)
C12N 9/00 (2013.01); C12N 9/22 (2013.01); C12N 15/907 (2013.01)
Citation (search report)
See references of WO 0138511A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0138511 A2 20010531; WO 0138511 A3 20020510; WO 0138511 A8 20020530; AU 1823901 A 20010604; CA 2394827 A1 20010531; DE 19956513 A1 20010621; EP 1240316 A2 20020918; JP 2003533176 A 20031111
DOCDB simple family (application)
EP 0011718 W 20001124; AU 1823901 A 20001124; CA 2394827 A 20001124; DE 19956513 A 19991124; EP 00977566 A 20001124; JP 2001540261 A 20001124