EP 1240318 A4 20041208 - EMBRYONIC OR STEM-LIKE CELLS PRODUCED BY CROSS SPECIES NUCLEAR TRANSPLANTATION
Title (en)
EMBRYONIC OR STEM-LIKE CELLS PRODUCED BY CROSS SPECIES NUCLEAR TRANSPLANTATION
Title (de)
DURCH SPEZIES-ÜBERGREIFENDE, NUKLEäRE TRANSPLANTATION HERGESTELLTE EMBRYONALE ODER STAMMZELLEN-ÄHNLICHE ZELLEN
Title (fr)
CELLULES SOUCHES OU EMBRYONNAIRES PRODUITES PAR TRANSPLANTATION NUCLEAIRE HETEROSPECIFIQUE
Publication
Application
Priority
- US 0012631 W 20000510
- US 46707699 A 19991220
Abstract (en)
[origin: WO0146401A1] Method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell, in conjunction with compatible cytoplasm and/or mitochondria (of same species as donor), are provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Nuclear transfer efficiency is enhanced by introduction of compatible cytoplasm or mitochondrial DNA (same species or similar to donor cell or nucleus). Also, nuclear transfer efficiency can further optionally be enhanced by genetically altering donor cells to inhibit apoptosis, by selecting for a specific cell cycle and/or by culturing under conditions that enhance embryonic growth and development.
IPC 1-7
IPC 8 full level
C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/87 (2006.01); C12N 15/873 (2010.01)
CPC (source: EP)
C12N 15/873 (2013.01)
Citation (search report)
- [XY] WO 9945100 A1 19990910 - UNIV MASSACHUSETTS [US]
- [E] WO 0119977 A1 20010322 - UNIV MASSACHUSETTS [US], et al
- [E] WO 0100793 A1 20010104 - HWANG WOO SUK [KR], et al
- [Y] LANZA R P ET AL: "HUMAN THERAPEUTIC CLONING", NATURE MEDICINE, NATURE PUBLISHING, CO, US, vol. 5, no. 9, September 1999 (1999-09-01), pages 975 - 977, XP000978948, ISSN: 1078-8956
- [A] MEIRELLES FV, SMITH LC.: "Mitochondrial genotype segregation during preimplantation development in mouse heteroplasmic embryos.", GENETICS., vol. 148, no. 2-98, February 1998 (1998-02-01), pages 877 - 883, XP002299553
- [A] BLERKOM VAN J ET AL: "MITOCHONDRIAL TRANSFER BETWEEN OOCYTES: POTENTIAL APPLICATIONS OF MITOCHONDRIAL DONATION AND THE ISSUE OF HETEROPLASMY", HUMAN REPRODUCTION, IRL PRESS, OXFORD, GB, vol. 13, no. 10, 1998, pages 2857 - 2868, XP001002313, ISSN: 0268-1161
- [A] BORDIGNON V ET AL: "TELOPHASE ENUCLEATION: AN IMPROVED METHOD TO PREPARE RECIPIENT CYTOPLASTS FOR USE IN BOVINE NUCLEAR TRANSFER", MOLECULAR REPRODUCTION AND DEVELOPMENT, LISSS, NEW YORK, NY, US, vol. 49, January 1998 (1998-01-01), pages 29 - 36, XP000910821, ISSN: 1040-452X
- [DY] KARNIKOVA L, URBAN F, MOOR R, FULKA J JR.: "Mouse oocyte maturation: the effect of modified nucleocytoplasmic ratio.", REPROD NUTR DEV., vol. 38, no. 6, December 1998 (1998-12-01), pages 665 - 670, XP008036540
- See references of WO 0146401A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0146401 A1 20010628; WO 0146401 A8 20010726; AU 4830900 A 20010703; AU 784731 B2 20060608; BR 0016561 A 20020910; CA 2394614 A1 20010628; CN 1425064 A 20030618; EP 1240318 A1 20020918; EP 1240318 A4 20041208; IL 150065 A0 20021201; IL 150065 A 20090922; JP 2004500066 A 20040108; MX PA02006093 A 20040823; NZ 519346 A 20040227
DOCDB simple family (application)
US 0012631 W 20000510; AU 4830900 A 20000510; BR 0016561 A 20000510; CA 2394614 A 20000510; CN 00818280 A 20000510; EP 00930498 A 20000510; IL 15006500 A 20000510; IL 15006502 A 20020606; JP 2001546899 A 20000510; MX PA02006093 A 20000510; NZ 51934600 A 20000510