EP 1240357 A1 20020918 - INCREMENTALLY TRUNCATED NUCLEIC ACIDS AND METHODS OF MAKING SAME
Title (en)
INCREMENTALLY TRUNCATED NUCLEIC ACIDS AND METHODS OF MAKING SAME
Title (de)
FORTSCHREITEND VERKÜRZTE NUKLEINSÄUREN UND VERFAHREN ZU DEREN HERSTELLUNG
Title (fr)
ACIDES NUCLEIQUES TRONQUES PROGRESSIVEMENT ET PROCEDES PERMETTANT DE LES PREPARER
Publication
Application
Priority
- US 0032114 W 20001116
- US 17252599 P 19991217
- US 57534500 A 20000519
- US 71846500 A 20001115
Abstract (en)
[origin: WO0175158A1] A series of methods that utilize the incremental truncation of nucleic acids are described to create a plurality of modified nucleic acids and hybrid polypeptides. A plurality of substantially all possible single base-pair deletions of a given nucleic acid sequence is created. A method of making shuffled incremental truncated nucleic acids, which is independent of nucleic acid sequence homology is also described, and shown in figure 1. These methods can be used in protein engineering, protein folding, protein evolution, and the chemical synthesis of novel hybrid proteins and polypeptides.
IPC 1-7
IPC 8 full level
C07K 14/00 (2006.01); C12N 15/01 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01)
CPC (source: EP US)
C12N 15/102 (2013.01 - EP US); C12N 15/1027 (2013.01 - EP US); C12N 15/1058 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0175158 A1 20011011; AU 1791701 A 20011015; CA 2393117 A1 20011011; EP 1240357 A1 20020918; EP 1240357 A4 20031029; JP 2004528801 A 20040924; US 2008227650 A1 20080918; US 2008227659 A1 20080918; US 7332308 B1 20080219; US 7820413 B2 20101026
DOCDB simple family (application)
US 0032114 W 20001116; AU 1791701 A 20001116; CA 2393117 A 20001116; EP 00980690 A 20001116; JP 2001573030 A 20001116; US 3367208 A 20080219; US 3369108 A 20080219; US 71846500 A 20001115